文献类型：期刊文献

英文题名：Effects of Florfenicol on nirS-Type Denitrification Community Structure of Sediments in an Aquatic Microcosm Model

作者：Zhang, Tengyue[1,2];Sun, Junying[3];Peng, Jinju[1];Ding, Yuexia[1];Li, Yang[1];Ma, Haotian[1];Yu, Mengbo[1];Ma, Yi[1]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Dept Vet Med, Zhanjiang 524088, Peoples R China;[2]Yangzhou Univ, Coll Vet Med, Yangzhou 225000, Peoples R China;[3]Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China

年份：2023

卷号：12

期号：8

外文期刊名：ANTIBIOTICS-BASEL

收录：SCI-EXPANDED(收录号：WOS:001055714700001)、、Scopus(收录号：2-s2.0-85169021090)、WOS

基金：This research was funded by the Natural Science Foundation of Guangdong Province, China(2023A1515012181); the Zhanjiang Science and Technology Bureau 2021 Provincial Science and Technology Special Funds ("Big Special + Task List") Competitive Allocation Project (2021A05231); and the Science and Technology Planning Project of Guangzhou city, China (202002030456, 20212100050).

语种：英文

外文关键词：florfenicol; nirS gene; denitrification community structure; sediment; aquatic microcosm

外文摘要：Florfenicol is one of the most widely used antibiotics in aquaculture and veterinary clinics because of its low side effects and strong bactericidal effect. A total of 45 similar to 60% of florfenicol is not absorbed by the animal body and accumulates in the aquatic environment through a variety of pathways, which affects denitrification. Indoor aquatic microcosm models were constructed and sediment samples were collected at different florfenicol concentrations (0.1, 1, 10, and 100 mg/L) on days 0, 7, 30, and 60 to extract the microbial genome DNA and determine the water properties. qPCR and amplicon sequencing were used to study the dynamic changes in the nirS gene and nirS-type denitrification community structure, diversity, and abundance, respectively. The results showed that high florfenicol stress influenced the sediment's physicochemical properties, reducing conductivity, alkaline dissolved nitrogen, and organic matter content. In addition, the abundance of nirS, a functional denitrification gene, increased obviously with increased florfenicol concentrations but decreased the diversity of nirS-type denitrification microorganisms. Proteobacteria was the dominant denitrifying phylum in the sediment. Our study provides a scientific basis for the rational use of florfenicol in aquaculture to maintain a healthy and stable microecological environment and also provides a preliminary understanding of the response characteristics of water denitrifying microorganisms to florfenicol exposure.