文献类型：期刊文献

英文题名：GC-TOF/MS-based metabolomics studies on the effect of protein sources in formulated diet for pearl oyster Pinctada fucata martensii

作者：Yang, Chuangye[1];Hao, Ruijuan[1];Du, Xiaodong[1,2];Wang, Qingheng[1,2];Deng, Yuewen[1,2];Sun, Ruijiao[3];Zheng, Zhe[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang 524088, Peoples R China;[3]Zhejiang Hengxing Food Co Ltd, Jiaxing 314100, Peoples R China

年份：2018

卷号：486

起止页码：139

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000425875900019)、、Scopus(收录号：2-s2.0-85038246220)、WOS

基金：This work was supported by the Science and Technology Department, Guangdong Province (grant number 2014A020208122); the Guangdong Marine and Fishery Bureau (grant numbers Z2014009 and B201601-Z10); the Department of Education of Guangdong Province (grant number 2014GKXM049); the Graduate education innovation program of Guangdong Ocean University (grant number 201720) and the China Agriculture Research System (grant number CARS-047).

语种：英文

外文关键词：Pinctada fucata martensii; Gas chromatography mass spectroscopy (GC-MS); Formulated diet; Metabolomics

外文摘要：Natural disasters and environmental pollution are the main problems for traditional offshore culture. Pearl oyster culture through industrial farming can prevent these difficulties, but food availability is limited. In this study, we formulated two diets, P3 and P5, with yeast powder and corn gluten, respectively, as major protein sources. Two experimental groups (EG1 and EG2) were separately fed with P3 and P5 diets indoors. After 45 days of feeding, GC-TOF/MS-based metabolomics of the hepatopancreas was performed to assess the effect of different formulated diets on pearl oyster (Pinctada fucata martensii) combined with growth performance. EG1 displayed significantly faster absolute growth rate and higher survival rate than EG2. In the metabolomic assay, 125 metabolites were identified by mass spectrum matching with a spectral similarity value (SV) > 700, and 35 were considered significantly different metabolites (SDMs) (VIP > 1 and P < 0.05). Pathway analysis indicated that the SDMs were involved in 34 pathways, including seven influential pathways (pathway impact value > 0.1). The impact values of phenylalanine, tyrosine, and tryptophan biosynthesis, ascorbate and aldarate metabolism, phenylalanine metabolism, inositol phosphate metabolism, tyrosine metabolism, sulfur metabolism, and cysteine and methionine metabolism were 0.500, 0.500, 0.308, 0.219, 0.207, 0.167, and 0.161, respectively. Further integrated key metabolic pathway analysis showed that EG2 exhibited lower capability for inositol phosphate metabolism, ascorbate and aldarate metabolism, phenylalanine metabolism, and tyrosine metabolism. This study suggested that yeast powder is a better protein source of formulated diet for pearl oysters than corn gluten.