文献类型：期刊文献

中文题名：远东拟沙丁鱼酶解产物的降血压效果评价及其ACE抑制肽的分离纯化与鉴定

英文题名：Antihypertensive effect of enzymatic hydrolysate from Sardinops melanosticta muscle on spontaneously hypertensive rats and identification of its angiotensin-converting enzyme(ACE) inhibitory peptide

作者：王晶晶[1];吉薇[1];吉宏武[1,2,3,4];苏伟明[1,2,3,4];李亚会[1];刘书成[1,2,3,4]

机构：[1]广东海洋大学,食品科技学院,广东湛江524088;[2]广东省水产品加工与安全重点实验室,广东湛江524088;[3]水产品深加工广东普通高校重点实验室,广东湛江524088;[4]广东省海洋食品工程技术研究中心,广东湛江524088

年份：2016

卷号：42

期号：4

起止页码：63

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：CSTPCD、、北大核心2014、CSCD2015_2016、北大核心、CSCD

基金：广东省高等院校学科建设项目(编号:2013CXZDA020);广东省产学研合作项目(编号:2013B090600155);广东海洋大学创新强校项目(编号:GDOU2013050314,GDOU2014050203)

语种：中文

中文关键词：远东拟沙丁鱼;酶解产物;血管紧张素转移酶(ACE)抑制肽;原发性高血压大鼠;分离纯化

外文关键词：Sardinops melanosticta; hydrolysate; angiotensin-converting enzyme（ACE）; SHRs; isolation and purification;

中文摘要：以远东拟沙丁鱼肌肉为原料,采用动物蛋白水解酶和风味蛋白酶联合水解制备其酶解产物(简称为SMMH),SMMH通过截留分子量分别为10 k Da和3 k Da超滤膜进行分离,获得3个组分,分别称为A(MW>10k Da)、B(3 k Da

外文摘要：Sardinops melanosticta muscle was hydrolyzed using a combination of animal protein hydrolysis enzyme and flavor enzyme to obtain its hydrolysate abbreviated as SMMH. SMMH was then ultrafiltered with 10 k Da and 3k Da cut-off membranes to yield 3 fractions named as A（ MW 〉10 k Da）,B（ 3 k Da〈 MW〈 10 k Da） and C（ MW〉3 k Da）. Results from measurement of their ACE inhibitory activity suggested that the fraction C exhibited the strongest ACE inhibitory activity in vitro. To further evaluate its antihypertensive effect,a single oral administration（ short term） or continuous administration for 28 d（ long term） of fraction C were carried out. Fraction C supplementation decreased the systolic blood pressure（ SBP） in a dose-dependent manner. With the increase of dose,the systolic blood pressure in SHRs significantly decreased. To identify novel ACE inhibitory peptide,the fraction C was then further separated by gel filtration,ion-exchange chromatography,and reversed phase high-performance liquid chromatography（ RP-HPLC）. Two novel ACE inhibitory peptides were isolated. The amino acid sequences were KVEPLP and PAL with IC50 value of 12. 2 μmol / L and 22. 9 μmol / L,respectively.