文献类型：期刊文献

英文题名：A GRAPHENE/ENZYME-BASED ELECTROCHEMICAL SENSOR FOR SENSITIVE DETECTION OF ORGANOPHOSPHORUS PESTICIDES

作者：Zhou, Nong[1];Li, Chengyong[1];Mo, Rijian[1];Zhang, Peng[1];He, Lei[1];Nie, Fanghong[1];Su, Weiming[1];Liu, Shucheng[1];Gao, Jing[1];Shao, Haiyan[1];Qian, Zhong-Ji[2];Ji, Hongwu[1]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod, Coll Food Sci & Technol,Guangdong Higher Educ Ins, Zhanjiang 524088, Peoples R China;[2]Pukyong Natl Univ, Dept Chem, Busan 608737, South Korea

年份：2016

卷号：23

期号：2

外文期刊名：SURFACE REVIEW AND LETTERS

收录：SCI-EXPANDED(收录号：WOS:000371723100008)、、EI(收录号：20155201728064)、Scopus(收录号：2-s2.0-84959532902)、WOS

基金：This work was supported by the National Natural Science Foundation of China (21405024), Foundation for Outstanding Young Teachers of Guangdong Ocean University (2014005), Guangdong Province Innovation School Project (2014KQNCX085, GDOU2014050247), Fund of Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (GDPKLAPPS1406).

语种：英文

外文关键词：Graphene oxide; Chitosan; carbaryl; trichlorfon; biosensor

外文摘要：A sensitive and fast sensor for quantitative detection of organophosphorus pesticides (OPs) is obtained using acetylcholinesterase (AChE) biosensor based on graphene oxide (GO)-chitosan (CS) composite film. This new biosensor is prepared via depositing GO-CS composite film on glassy carbon electrode (GCE) and then assembling AChE on the composite film. The GO-CS composite film shows an excellent biocompatibility with AChE and enhances immobilization efficiency of AChE. GO homogeneously disperses in the GO-CS composite films and exhibits excellent electrocatalytic activity to thiocholine oxidation, which is from acetylthiocholine catalyzed by AChE. The results show that the inhibition of carbaryl/trichlorfon on AChE activity is proportional to the concentration of carbaryl/trichlorfon. The detection of linear range for carbaryl is from 10 nM to 100 nM and the correlation coefficients of 0.993. The detection limit for carbaryl is calculated to be about 2.5 nM. In addition, the detection of linear range for trichlorfon is from 10 nM to 60 nM and the correlation coefficients of 0.994. The detection limit for trichlorfon is calculated to be about 1.2 nM. This biosensor provides a new promising tool for trace organophosphorus pesticide detection.