文献类型：期刊文献

英文题名：Functional characterization of a protein inhibitor of activated STAT (PIAS) gene in Litopenaeus vannamei

作者：Zhang, Shuang[1,2,3];Li, Chaozheng[4,5];Wang, Wei[1];Wang, Chenggui[1];Sun, Chengbo[1];Chan, Siuming[1];Shi, Lili[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang, Peoples R China;[3]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Tech, Zhanjiang, Peoples R China;[4]Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Marine Sci, Guangzhou, Guangdong, Peoples R China;[5]Southern Lab Ocean Sci & Engn, Zhuhai, Guangdong, Peoples R China

年份：2019

卷号：94

起止页码：417

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000496892400045)、、Scopus(收录号：2-s2.0-85072279013)、WOS

基金：This study was supported by Science and Technology Directorate Program of Guangzhou City of China (201804020013); National Natural Science Foundation of China (31702377); Free application Fund of Natural Science Foundation of Guangdong Province, China (2018A030313963; 2016A030313757).

语种：英文

外文关键词：Protein inhibitor of activated STAT; Signal transducer and activator of transcription; Litopenaeus vannamei; WSSV

外文摘要：Protein inhibitor of activated STAT (PIAS) plays a critical role in the feedback modulation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway as a negative regulator in mammals and Drosophila, but the function of PIAS in crustaceans is still unclear. In this study, a PIAS termed LvPIAS was cloned and characterized from Litopenaeus vannamei. The full length of LvPIAS was 3065 bp, including a 2361 bp open reading frame (ORF) coding for a protein of 786 aa. LvPIAS expression was most abundant in muscle and could respond to the challenge of LPS, Vibrio parahaemolyticus, Staphhylococcus aureus, Poly I: C and white spot syndrome virus (WSSV). LvPIAS could be induced by the transcription factor LvSTAT, but LvPIAS could inhibit the transcriptional activity of LvSTAT to the LvPIAS promoter conversely, which indicated that there was a negative feedback loop between LvSTAT and LvPIAS. Furthermore, RNAi-mediated knockdown of LvPIAS shrimps showed higher survival rate to WSSV infection than those in the control group (dsGFP injection), suggesting that LvPIAS may play a negatively role against WSSV infection.