文献类型：期刊文献

英文题名：Cloning and functional analysis of the calreticulin gene from the scleractinian coralGalaxea astreata

作者：Huang, Yuanjia[1];Zhang, Yanping[1];Peng, Huipai[1];Yuan, Jigui[1];Liu, Li[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Key Lab Aquaculture South China Sea Aquat Econ An, Guangdong Higher Educ Inst, Zhanjiang 524025, Peoples R China

年份：2020

卷号：39

期号：6

起止页码：58

外文期刊名：ACTA OCEANOLOGICA SINICA

收录：SCI-EXPANDED(收录号：WOS:000544300200007)、、Scopus(收录号：2-s2.0-85087122010)、WOS

语种：英文

外文关键词：Galaxea astreata; calreticulin; bacterial agglutination

外文摘要：Calreticulin (CRT) is a highly conserved Ca2+-binding protein and chaperone in the endoplasmic reticulum, which mainly participates in adjusting calcium level and directing proper conformation of proteins. Here, we cloned thecrtgene of the scleractinian coralGalaxea astreata, namedGacrt, and analysed its ability to drive bacterial agglutination. The full-lengthGacrtcDNA consisted of 1 792 nucleotides and contained a 77 bp 5 ' untranslated region (UTR), a 380 bp 3 ' UTR and a 1 335 bp open reading frame (ORF) that encoded a 444 amino acid protein. The deduced peptide possessed a signal peptide domain, an endoplasmic reticulum retrieval signal sequence (KDEL), two potential calreticulin family signature motifs and a set of triplicate repeats. We also found that the recombinant GaCRT protein could promote agglutination of both the Gram-positive bacteriumMicrococcus luteusand the Gram-negative bacteriumEscherichia coli.These results show that the GaCRT protein can enhance bacterial agglutination, hinting that GaCRT is an immune-relevant molecule involved in host defense against bacterium.