文献类型：期刊文献

英文题名：Transcriptional and Biochemical Alterations in Zebrafish Eleuthero-Embryos (Danio rerio) After Exposure to Synthetic Progestogen Dydrogesterone

作者：Shi, Wen-Jun[1];Ying, Guang-Guo[1];Huang, Guo-Yong[1];Liang, Yan-Qiu[1,2];Hu, Li-Xin[1];Zhao, Jian-Liang[1];Zhang, Jin-Na[1]

机构：[1]Chinese Acad Sci, Guangzhou Inst Geochem, CAS Res Ctr PRD Environm Pollut & Control, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China

年份：2017

卷号：99

期号：1

起止页码：39

外文期刊名：BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000403502200007)、、WOS

基金：The authors would like to acknowledge the financial support from the National Natural Science Foundation of China (41273119, and U1401235), National Water Pollution Control Program (2014ZX07206-005) and GIG CAS (IS-2348).

语种：英文

外文关键词：Dydrogesterone; Zebrafish; Transcription; Biochemical alteration

外文摘要：Little information has so far been known on the effects of synthetic progestogen dydrogesterone (DDG) in organisms like fish. This study aimed to investigate the effects of DDG on the transcriptional and biochemical alterations in zebrafish eleuthero-embryos. Zebrafish eleuthero-embryos were analyzed for the transcriptional alterations by real-time quantitative PCR (RT-qPCR) and biochemical changes by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FITR) after 144 h exposure to DDG. The results of qPCR analysis showed that DDG exposure significantly suppressed the transcriptions of target genes involved in hypothalamic-pituitary-thyroid (HPT) axis, while it induced the expression of target genes mRNA belonging to hypothalamic-pituitary-gonad (HPG) axis. In addition, ATR-FTIR spectroscopy analysis showed that the biochemical alterations of protein, nucleic acid and lipid were observed following DDG treatment. The finding from this study suggests that DDG exposure could have potential multiple effects in fish.