文献类型：期刊文献

中文题名：香蕉增殖培养培养基配方的优化

英文题名：Optimization of medium for banana proliferation in vitro

作者：丰锋[1];叶春海[1];王耀辉[1]

机构：[1]广东海洋大学农学院,广东湛江524088

年份：2007

卷号：24

期号：3

起止页码：397

中文期刊名：果树学报

外文期刊名：Journal of Fruit Science

收录：CSTPCD、、北大核心2004、CSCD_E2011_2012、北大核心、CSCD

基金：广东省科技厅农业攻关项目(2004B20901006);湛江市科技招标项目(0409006)。

语种：中文

中文关键词：香蕉;正交设计;培养基优化;有效芽指数

外文关键词：Banana ; Orthogonal device; Optimization of medium ; Available bud index

中文摘要：以香蕉品种巴西为试材,利用正交试验设计方法优化香蕉继代培养的培养基配方,提出有效芽指数的概念并用以评价继代培养的效果。结果表明:(1)40g/L的蔗糖既有利于不定芽的增殖,又能控制带叶鞘芽的比例,提高有效芽指数。(2)组合6-BA1.0mg/L+Ad5.0mg/L+NAA0.1mg/L+40g/L蔗糖增殖有效芽指数(2.7436)最高,组合6-BA3.0mg/L+NAA0.1mg/L+60g/L蔗糖生根有效芽指数(1.5588)最高。

外文摘要：Experiment was conducted with Brazil banana cuhivar（Musa AAA cavendish subgroup cv. Brazil）for optimization of medium constituents for in vitro proliferation by orthogonal device and available bud index was used for evaluating the effects of proliferation. The results showed that 40 g/L sugar not only facilitated the proliferation of buds, but also controlled the proportion of buds with leaf sheath, and increased available bud index. The highest available proliferation bud index （2.743 6） was obtained with the medium contained 6-BA 1.0 mg/L, Ad 5.0 mg/L, NAA 0.1 mg/L, sugar 40 g/L; The highest available rooted bud index （1.558 8） was obtained with the medium contained 6-BA 3.0 mg/L, NAA 0.1 mg/L and sugar 60 g/L.