文献类型：期刊文献

英文题名：The occurrence of Toxocara malaysiensis in cats in China, confirmed by sequence-based analyses of ribosomal DNA

作者：Li M.-W.[1,2];Zhu X.-Q.[2];Gasser R.B.[3];Lin R.-Q.[2];Sani R.A.[4];Lun Z.-R.[5];Jacobs D.E.[6]

机构：[1]S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Dept Vet Med, Zhanjiang 524088, Guangdong, Peoples R China;[3]Univ Melbourne, Dept Vet Sci, Werribee, Vic 3030, Australia;[4]Univ Pertanian Malaysia, Fac Vet Med, Serdang 43400, Selangor DE, Malaysia;[5]Zhongshan Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China;[6]Univ London, Univ London Royal Vet Coll, Dept Pathol & Infect Dis, Hatfield AL9 7TA, Herts, England

年份：2006

卷号：99

期号：5

起止页码：554

外文期刊名：PARASITOLOGY RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000241385100007)、、Scopus(收录号：2-s2.0-33748354230)、WOS

基金：Acknowledgements Project support was provided to XQZ by grants from the China National Science Funds for Distinguished Young Scientists (Grant no. 30225033), and from the Special Funds for the Training of Ph.D. Students from the Ministry of Education, China (Grant no. 20040564008).

语种：英文

外文摘要：Non-isotopic polymerase chain reaction (PCR)based single-strand conformation polymorphism and sequence analyses of the second intemal transcribed spacer (ITS-2) of nuclear ribosomal DNA (rDNA) were utilized to genetically characterise ascaridoids from dogs and cats from China by comparison with those from other countries. The study showed that Toxocara canis, Toxocara cati, and Toxascaris leonina from China were genetically the same as those from other geographical origins. Specimens from cats from Guangzhou, China, which were morphologically consistent with Toxocara malaysiensis, were the same genetically as those from Malaysia, with the exception of a polymorphism in the ITS-2 but no unequivocal sequence difference. This is the first report of T malaysiensis in cats outside of Malaysia (from where it was originally described), supporting the proposal that this species has a broader geographical distribution. The molecular approach employed provides a powerful tool for elucidating the biology, epidemiology, and zoonotic significance of T malaysiensis.