文献类型：期刊文献

英文题名：Structural characterization of a water-soluble acidic polysaccharide CSP-IV with potential anticoagulant activity from fruit pulp of Clausena lansium (Lour.) Skeels Guifei

作者：Wu, Jiayi[1];Zheng, Wenyan[1];Luo, Ping[1];Lin, Zhen[1];Li, Fangping[1];Liang, Linlin[1];Liu, Huazhong[1]

机构：[1]Guangdong Ocean Univ, Coll Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2024

卷号：254

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001113709200001)、、EI(收录号：20234615053131)、Scopus(收录号：2-s2.0-85176215098)、WOS

基金：Funding The study was supported by the Guangdong Basic and Applied Basic Research Foundation (No. 2022A1515012091) and Special Project in Key Fields of Guangdong Universities (No. 2022ZDZX2025) .

语种：英文

外文关键词：Wampee fruit pulp polysaccharides; Anticoagulant activity; Molecular characteristics

外文摘要：Four main water-soluble wampee fruit pulp polysaccharides, named CSP-I, CSP-II, CSP-III and CSP-IV, were isolated from Clausena lansium (Lour.) Skeels Guifei, therein CSP-IV content was higher than the others. All components possess certain anticoagulant activity demonstrated by prolonged activated partial thromboplastin time, especially CSP-IV, which suggests that CSP-IV plays anticoagulant effect through disturbing intrinsic coagulation pathway. The wampee polysaccharide CSP-IV with Mw of 510.1 kDa was mainly composed of Gal, Ara and GalA. Backbone of CSP-IV contains Gal, Ara and GalA, two kinds of side chains contain one monosaccharide Gal or Ara, both branch on Gal residue of backbone. CSP-IV has no the conformation of triple helix demonstrated by Congo red test. These results showed that CSP-IV is an acidic polysaccharide with potential anticoagulant activity via targeting intrinsic coagulation pathway.