文献类型：期刊文献

英文题名：Comparison of protective efficacy between two DNA vaccines encoding DnaK and GroEL against fish nocardiosis

作者：Chen, Jianlin[1,2,3,4];Wang, Wenji[1,2,3,4];Hou, Suying[1,2,3,4];Fu, Weixuan[1,2,3];Cai, Jia[2,4];Xia, Liqun[1,2,3,5];Lu, Yishan[1,2,3,4,5]

机构：[1]Guangdong Ocean Univ, Shenzhen Inst, 3 Binhai 2nd Rd, Shenzhen 518108, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang, Guangdong, Peoples R China;[3]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen, Guangdong, Peoples R China;[4]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Guangdong, Peoples R China;[5]Shenzhen Publ Serv Platform Evaluat Marine Econ A, Shenzhen, Guangdong, Peoples R China

年份：2019

卷号：95

起止页码：128

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000503099200011)、、Scopus(收录号：2-s2.0-85073476460)、WOS

基金：We are grateful to all the laboratory members for their constructive suggestions to improve the manuscript. This work was supported by Natural Science Foundation of Guangdong Province (2017A030313179), Research Projects of Guangdong Ocean University's Top-ranking Discipline Construction (231419017, 231419013), Shenzhen Science and Technology Project (JCYJ20180306173022502, JCYJ20180507183240459), Shenzhen Dapeng New District special fund for industry development (PT20190106) and Natural Science Foundation of Guangdong Ocean University (C17377, C13454, Y14289).

语种：英文

外文关键词：Fish nocardiosis; Nocardia seriolae; Molecular chaperone DnaK; Molecular chaperone GroEL; DNA vaccine

外文摘要：Fish nocardiosis is a chronic granulomatous bacterial disease mainly caused by three pathogenic bacteria, including Nocardia seriolae, N. asteroids and N. salmonicida. Molecular chaperone DnaK and GroEL were identified to be the common antigens of the three pathogenic Nocardia species in our previous studies. To evaluate the immune protective effect of two DNA vaccines encoding DnaK or GroEL against fish nocardiosis, hybrid snakehead were vaccinated and the immune responses induced by these two vaccines were comparatively analyzed. The results suggested it needed at least 7 d to transport DnaK or GroEL gene from injected muscle to head kidney, spleen and liver and stimulate host's immune system for later protection after immunization by DNA vaccines. Additionally, non-specific immunity parameters (serum lysozyme (LYZ), peroxidase (POD), acid phosphatase (ACP), alkaline phosphatase (AKP) and superoxide dismutase (SOD) activities), specific antibody (IgM) production and immune-related genes (MHCI alpha, MHCII alpha, CD4, CD8 alpha, IL-1 beta and TNF alpha) were used to evaluate the immune responses induced in vaccinated hybrid snakehead. It proved that all the above-mentioned immune activities were significantly enhanced after immunization with these two DNA vaccines. The protective efficacy of pcDNA-DnaK and pcDNA-GroEL DNA vaccines, in terms of relative percentage survival (RPS), were 53.01% and 80.71% respectively. It demonstrated that these two DNA vaccines could increase the survival rate of hybrid snakehead against fish nocardiosis, albeit with variations in immunoprotective effects. Taken together, these results indicated that both pcDNA-DnaK and pcDNA-GroEL DNA vaccines could boost the innate, humoral and cellular immune response in hybrid snakehead and show highly protective efficacy against fish nocardiosis, suggesting that DnaK and GroEL were promising vaccine candidates. These findings will promote the development of DNA vaccines against fish nocardiosis in aquaculture.