文献类型：期刊文献

英文题名：miR-21-3p Targets Elovl5 and Regulates Triglyceride Production in Mammary Epithelial Cells of Cow

作者：Li, Xiaohui[1];Jiang, Ping[1];Yu, Haibin[1];Yang, Yuwei[1];Xia, Lixin[1];Yang, Runjun[1];Fang, Xibi[1];Zhao, Zhihui[1,2]

机构：[1]Jilin Univ, Dept Anim Sci, Xi An Rd 5333, Changchun 130062, Jilin, Peoples R China;[2]Guangdong Ocean Univ, Dept Anim Sci, Haida Rd 1, Mazhang Dist 524088, Zhanjiang, Peoples R China

年份：2019

卷号：38

期号：4

起止页码：352

外文期刊名：DNA AND CELL BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000463614100009)、、WOS

基金：We are very grateful to Mengyan Wang of our lab for helping during the revision process. This work was supported by the National Major Special Project on New Varieties Cultivation for Transgenic Organisms (2016ZX08009003-006), the National Natural Science Foundation of China (no. 31372278 and no. 31672389), the National High Technology Research and Development Program (863 Program, no. 2013AA102505), the Jilin Scientific and Technological Development Program (no. 20170519014JH), and the Jilin province industrial technology research and development program (2016C032).

语种：英文

外文关键词：miR-21-3p; Elovl5; triglycerides; MECs

外文摘要：MicroRNAs (miRNAs) can regulate a variety of biological functions such as fat and cholesterol synthesis, translocation, and utilization by regulating degradation or by inhibiting target mRNA translation. In this study, the target gene of miR-21-3p was analyzed through bioinformatics, which predicted Elovl5. Furthermore, miR-21-3p was verified for regulation of triglyceride expression. The results showed that luciferase activity was significantly lower in cells cotransfected with miR-21-3p and Elovl5 reporter vector, pmiR-RB-REPORT-Elovl5-WT, than in cells with miR-21-3p and reporter control vectors, pmiR-RB-REPORT-Elovl5-mut and pmiR-RB-REPORT-Elovl5-si, indicating that target sites exist in the 3 ' UTR of Elovl5. Further analysis using qPCR and Western blotting showed that the expression of miR-21-3p negatively correlated with the levels of Elovl5 mRNA and protein, suggesting that miR-21-3p might play an important role in regulating Elovl5 gene expression. Finally, transfection with bta-miR-21-3p mimics, bta-miR-21-3p inhibitor, or miRNA-ShNC in mammary epithelial cells suggested that bta-miR-21-3p promotes triglyceride production, which might be attributed to the expression of the target gene Elovl5.