文献类型：期刊文献

英文题名：A comprehensive profile of the tilapia (Oreochromis niloticus) circular RNA and circRNA-miRNA network in the pathogenesis of meningoencephalitis of teleosts

作者：Fan, Bolin[1];Chen, Funuan[1];Li, Yuan[1];Wang, Zhongliang[1];Wang, Zhiwen[1];Lu, Yishan[1,2];Wu, Zaohe[1];Jian, Jichang[1,3,4];Wang, Bei[1,3,4]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim Guangdong Higher E, Coll Fishery, Zhanjiang, GD, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, GD, Peoples R China;[3]Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China;[4]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China

年份：2019

卷号：15

期号：3

起止页码：233

外文期刊名：MOLECULAR OMICS

收录：SCI-EXPANDED(收录号：WOS:000474156900004)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 31702386), International Cooperation Science & Technology Planning Project of Guangdong Province of China (Grant No. 2017A050501037). We are especially grateful for the critical comments from the anonymous reviewers. The authors also thank Genedenovo Biothchnology Co., Ltd (Guangzhou, China) for circRNA sequence and assisting in the revision of this manuscript.

语种：英文

外文摘要：The pathogenesis of tilapia meningoencephalitis is still unclear, where the involvement of circRNA is considered for its active role as a `` miRNA sponge''. Therefore, we aimed to investigate the profile of circRNA in tilapia meningoencephalitis further by constructing the circRNA-miRNA network for in-depth mechanism exploration. Briefly, a nile tilapia model of meningitis was established by injecting Streptococcus agalactiae (1.0 x 10(7) cfu per mL) and we evaluated the infected tilapia brain for the expression profile of circRNAs, their potential functions and their correlation with genes involved in inflammatory pathways. A total of 11 263 circRNAs were identified from RNA sequencing (RNA-seq) data in nile tilapia (Oreochromis niloticus), a commercially important fish in China and East Asia. GO and KEGG analyses revealed that the biological functions of genes hosting the circRNAs were enriched in the progression of metabolism and binding. Notably, we found that 99% circRNAs in tilapia had abundant miRNA-binding sites and a total of 2136 of the identified circRNAs had two to six miRNA-binding sites. Six circRNAs were validated by qRT-PCR and the final circRNA-miRNA network was constructed. This is the first report of comprehensive identification of O. niloticus circRNAs being differentially regulated in the brain in normal conditions relating to S. agalactiae infection. This work will shed novel light on gene expression mechanisms under disease conditions and may identify circRNAs as new biomarkers for meningoencephalitis and neurodegenerative disorders.