文献类型：期刊文献

英文题名：Hemopexin as an acute phase protein regulates the inflammatory response against bacterial infection of Nile tilapia (Oreochromis niloticus)

作者：Yin, Xiaoxue[1]; Li, Xiaoyu[2,4]; Chen, Nuo[1]; Mu, Liangliang[1]; Wu, Hairong[1]; Yang, Yanjian[1]; Han, Kailiang[1]; Huang, Yu[3]; Wang, Bei[3]; Jian, Jichang[3]; Wang, Anli[1]; Ye, Jianmin[1]

机构：[1] School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, China; [2] College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100124, China; [3] Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Ocean University, Zhanjiang, 524088, China; [4] State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Life Omics, Beijing, 102206, China

年份：2021

卷号：187

起止页码：166

外文期刊名：International Journal of Biological Macromolecules

收录：EI(收录号：20242616379254)、Scopus(收录号：2-s2.0-85111051266)

语种：英文

外文关键词：Polymerase chain reaction - Recombinant proteins

外文摘要：Hemopexin, a high affinity heme-binding protein is widely involved in variety physiological and pathological processes. It is an important acute phase response protein, and is important in regulating the inflammatory response. In this study, the open reading frame of Nile tilapia hemopexin (OnHpx) gene was amplified. The expression pattern of OnHpx in natural and bacterial challenged tilapia tissues were analyzed through RT-qPCR. The results indicated the OnHpx was most abundant in liver, and increased significantly in liver, spleen, head kidney and peripheral blood after bacterial challenge. Furthermore, the OnHpx mRNA was also significantly up-regulated in monocytes/macrophages and hepatocytes under the stimulation of S. agalactiae or A. hydrophila. In addition, the recombinant OnHpx protein could effectively reduce the bacteria proliferation and alleviate the inflammatory reaction caused by bacteria. Moreover, the (r)OnHpx also regulated the respiratory burst of monocytes/macrophages and played an important role in the antioxidant process. To our knowledge, these results provide the first evidence on the antibacterial and anti-inflammatory response mechanism of Hpx in early vertebrates. This brings new insights about the understanding of the evolutionary origins and ancient roles of the Hpx in the innate immune defense. ? 2021