文献类型：期刊文献

英文题名：Selection of reference genes for gene expression studies in PBMC from Bama miniature pig under heat stress

作者：Ju Xiang-Hong[1,2];Yong Yan-Hong[2];Xu Han-Jin[3];An Li-Long[3];Xu Ying-Mei[3];Jiao Pei-Rong[1];Liao Ming[1]

机构：[1]S China Agr Univ, Key Lab Anim Dis Control & Prevent, Minist Agr, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Dept Vet Med, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Dept Anim Sci, Zhanjiang 524088, Peoples R China

年份：2011

卷号：144

期号：1-2

起止页码：160

外文期刊名：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

收录：SCI-EXPANDED(收录号：WOS:000297443000021)、、Scopus(收录号：2-s2.0-82555172427)、WOS

语种：英文

外文关键词：Bama miniature pig; Heat stress; Reference gene; Toll-like receptor 2

外文摘要：Heat stress decreases immune function and increases disease susceptibility in stressed animals, which are important factors for industry and public health. We investigated the molecular mechanisms underlying heat stress by profiling the expression of target genes involved in the cellular response in the blood of Bama miniature pigs (Sus scrora domestica) over 21 days with the use of quantitative RT-PCR (qRT-PCR). Reliable standards were established for the normalization of qRT-PCR. Six potential reference genes were ranked by their stability using the geNorm and NormFinder programs. Ribosomal protein L4 (RPL4) and TATA-box-binding protein (TBP) ranked as the two most stably expressed genes, except on day 21 when beta-2-microglobulin (B2M) was the most stable. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and ribosomal RNA 18S (18SRNA) were discarded as reference genes due to their unstable expression patterns. When B2M and TBP genes were selected as standards in combination, rather than GAPDH, a significant upregulation in Toll-like receptor 2 (TLR2) expression was observed after 21 consecutive days of heat stress. These findings suggest that selection of an ideal reference gene is a key step in comparison of transcription profiles in Bama miniature pigs. (C) 2011 Elsevier B.V. All rights reserved.