文献类型：期刊文献

英文题名：Molecular and functional characterization of CD59 from Nile tilapia (Oreochromis niloticus) involved in the immune response to Streptococcus agalactiae

作者：Gan, Zhen[1,2,3];Wang, Bei[1,2,3];Zhou, Wei[1,2,3];Lu, Yishan[1,2,3];Zhu, Weiwei[1,2,3];Tang, Jufen[1,2,3];Jan, JiChang[1,2,3];Wu, Zaohe[2,3]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524025, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China;[3]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Anim, Zhanjiang 524025, Peoples R China

年份：2015

卷号：44

期号：1

起止页码：50

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000353743900007)、、WOS

基金：We thank all the laboratory members for their critical reviews and comments on this manuscript. We are especially grateful for the critical comments from the anonymous reviewers. This work was supported by research grants (No. 31302226, No. 20128020308009) from the National Natural Science Foundation of China and Science & Technology Planning Project of Guangdong Province of China.

语种：英文

外文关键词：Oreochromis niloticus; CD59; Streptococcus agalactiae; Complement-inhibitory activity; Antimicrobial activity

外文摘要：CD59, the major inhibitor of membrane attack complex, plays a crucial role in regulation of complement activation. In this paper, a CD59 gene of Nile tilapia, Oreochromis niloticus (designated as On-CD59) was cloned and its expression pattern under the stimulation of Streptococcus agalactiae was investigated. Sequence analysis showed main structural features required for complement inhibitory activity were detected in the deduced amino acid sequence of On-CD59. In healthy Nile tilapia, the On-CD59 transcripts could be detected in all the examined tissues, with the most abundant expression in the brain. When immunized with inactivated S. agalactiae, there was a clear time-dependent expression pattern of On-CD59 in the skin, brain, head kidney, thymus and spleen, with quite different kinetic expressions. The assays for the complement inhibitory activity suggested that recombinant On-CD59 protein had a species-selective inhibition of complement. Moreover, our works showed that recombinant On-CD59 protein may possess both binding activities to PGN and LTA and inhibiting activity of S. agalactiae. These findings indicated that Ors-CD59 may play important roles in the immune response to S. agalactiae in Nile tilapia. (C) 2015 Elsevier Ltd. All rights reserved.