文献类型：期刊文献

英文题名：Pentapeptide AYP from Isochrysis Zhanjiangensis Exhibits Antiangiogenic Activity in HT1080 Cells and HUVECs by Suppressing Migration and Invasion In Vitro

作者：Tang, Yanfei[1];Yang, Shengtao[1,2,3];Lin, Liyuan[1];Zheng, Zhaowan[1];Sun, Shengli[1];Zhou, Chunxia[1];Hong, Pengzhi[1];Qian, Zhong-Ji[1,2]

机构：[1]Guangdong Ocean Univ, Shenzhen Inst Guangdong Ocean Univ, Coll Food Sci & Technol, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524025, Peoples R China;[3]Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Key Lab Trop Crop Prod Proc Minist Agr & Rural Aff, Zhanjiang 524001, Guangdong, Peoples R China

年份：2022

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000829817600001)、、WOS

基金：The research was funded by the 2020 Shenzhen International Scientific and Technological Cooperation R&D Project (GJHZ20190823111601682) and the Natural Science Foundation of Guangdong Province (2020A1515011075). Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation ("Climbing Program" Special Funds, pdjh2022a0232). The supported by Natural Science Foundation of Hainan Province of China (Grant Number 322QN381), the Development Project about Marine Economy Demonstration of Zhanjiang City (XM-202008-01B1) and Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang, ZJW-2019-07).

语种：英文

外文关键词：Isochrysis zhanjiangensis; pentapeptide; tumor angiogenesis; inflammation

外文摘要：Microalgae are important biological sources of marine active peptides and renewable biological resources. Isochrysis zhanjiangensis has been widely used in biological ultrafiltration membranes and aquaculture. However, there are relatively few studies on its component structure and diverse activities. In this study, the mechanism of action of previously isolated pentapeptides (AYP, Ala-Tyr-Ala-Pro-Glu) on inflammation and tumor angiogenesis was evaluated. The results showed that AYP could effectively inhibit the invasion and migration of human umbilical vein endothelial cells (HUVECs) and HT1080 cells by downregulating the expression of MMP-2/-9, independent of cytotoxicity. Especially after 100 mu M AYP treatment, the ability to inhibit migration was about 67.7% +/- 1.9 for HT1080 cells and 63.6% +/- 1.3 for HUVECs, respectively. In addition, the activity of iNOS and COX-2 was decreased by inhibiting the oversecretion of VEGF in HT1080 cells induced by CoCl(2 )and the activation of VEGFR-2 in HUVECs and by regulating PI3K/AKT and Ras/MAPK signaling pathways. It can prevent inflammation and block tumor angiogenesis. Therefore, AYP is expected to become a drug or functional food to prevent and treat tumor angiogenesis.