文献类型：期刊文献

英文题名：Integrated transcriptomic and metabolomic analysis reveals the causes of mass mortality in juvenile pearl oysters (Pinctada maxima)

作者：Liu, Jinfang[1];Su, Qin[1];Yang, Chuangye[1];Luo, Junpeng[1];Hao, Ruijuan[2];Liao, Yongshan[6];Mkuye, Robert[1];Wang, Qingheng[1,3,4,5,6];Deng, Yuewen[1,3,4,5,6]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhanjiang, Zhanjiang 524088, Peoples R China;[3]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China;[4]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[5]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[6]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang, Peoples R China

年份：2025

卷号：55

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS

收录：SCI-EXPANDED(收录号：WOS:001436687000001)、、Scopus(收录号：2-s2.0-85218850650)、WOS

基金：This work was supported by Guangdong Basic and Applied Basic Research Foundation (Grant No. 2023A1515011769 and 2022A1515010590) , National Key Research and Development Program of China (Grant No. 2022YFD2401303) , Department of Education of Guangdong Province (Grant No. 2024ZDZX4061 and 2021KCXTD026) , the Fund of the Key Laboratory of Tropical Marine Ecosystem and Bio-resource, MNR (Grant No. 2021QN08) , and the earmarked fund for CARS-49. We are very grateful to Marine Pearl Science and Technology Backyard in Leizhou of Guangdong for collecting samples. The authors would like to thank TopEdit ( www.topeditsci.com ) for its linguistic assistance during the preparation of this manuscript. Transcriptomic and metabolomic analysiswas assisted by Biotree Biotech Co., Ltd. (Shanghai, China) .

语种：英文

外文关键词：Pinctada maxima; Mass mortality; Metabolome; Transcriptome

外文摘要：Pinctada maxima is a pearl oyster species producing large, high-quality marine pearls. However, juvenile mortality (shell length < 5 cm) in this species adversely affects commercial pearl production. Understanding the molecular mechanism and genes related to mass mortality will help mitigate this problem. Therefore, the present study investigated the transcriptomic and metabolic differences between pearl oysters during high mortality (HM) and after this period (PD) to shed light on the causes of juvenile mass mortality. Initial analysis of biochemical parameters revealed that protease, alpha-amylase, and catalase activities in the hepatopancreatic tissues of pearl oysters at the HM stage were significantly lower than at the PD stage. Conversely, glutathione and lysozyme contents, and superoxide dismutase, acid phosphatase, alkaline phosphatase activities were notably higher at the HM stage than at the PD stage. Metabolomic analysis identified 98 metabolites in the adductor muscle significantly different between the two stages, which enriched glycerophospholipid metabolism, glutathione metabolism, arachidonic acid metabolism, oxidative phosphorylation, and neuroactive ligand-receptor interaction pathways. Transcriptome analysis identified 677 differentially expressed genes in the adductor muscle between these stages, which enriched neuroactive ligand-receptor interaction, glutathione metabolism, and ECM-receptor interaction pathways. Finally, an integrated analysis of the metabolome and transcriptome suggested that pearl oysters at the HM stage experience oxidative stress, activate immune-related genes, and exacerbate the low energy status. These findings on the causes of mass mortality lay a theoretical foundation for improving the survival rate of juveniles and advancing the industrialization of P. maxima.