文献类型：期刊文献

英文题名：Genetic diversity and genetic structure in successive mass selected generations of tetraploid Pacific oysters (Crassostrea gigas), diploid Portuguese oysters (C. angulata) and their allotriploid oysters

作者：Ma, Haitao[1,2,3,4,5,6,7];Qin, Yanping[1,2,3,4,5,6,7];Zhang, Yinjie[8];Wan, Weitao[1,2,3,4,5,6,7];Huang, Jingyue[8];Mi, Na[1,2,3,4,5,6,7];Zhao, Zhen[8];Wang, Zhongyu[8];Li, Jiangwei[8];Li, Jun[1,2,3,4,5,6,7];Yu, Ziniu[1,2,3,4,5,6,7];Zhang, Yuehuan[1,2,3,4,5,6,7]

机构：[1]Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Peoples R China;[2]Agrotech Extens Ctr Guangdong Prov, Guangzhou 510145, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518120, Peoples R China;[4]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519015, Peoples R China;[5]Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China;[6]Chinese Acad Sci, Sanya Inst Ocean Ecoenvironm Engn, Hainan Prov Key Lab Trop Marine Biol Technol, Trop Marine Biol Res Stn Hainan, Sanya 572024, Peoples R China;[7]Chinese Acad Sci, Daya Bay Marine Biol Res Stn, Shenzhen 518124, Peoples R China;[8]Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Peoples R China

年份：2025

卷号：596

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001358579400001)、、Scopus(收录号：2-s2.0-85208664842)、WOS

基金：This research was supported by Guangdong Provincial Key Research and Development Program (2021B0202020003); Research on breeding technology of candidate species for Guangdong Modern Marine Ranching (2024-MRB-00-001); Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (No.SML2023SP201); Hainan Provincial Key R&D Programme (ZDYF2024XDNY175); Guangdong Basic and Applied Basic Research Foundation (2023A1515010944; 2022A1515010203); Guangdong Science and Technology Plan Programme (2024B1212050006); the Open Foundation of the State Key Laboratory of Loess and Quaternary Geology (SKLLQG2213); the Project of Sanya Yazhou Bay Science and Technology City; National Key Research and Development Program of China (2023YFD2401704; 2022YFC3103403; 2021YFC3100504); Guangzhou Science and Technology Project (202206010133; 2023B03J00165); the National Science Foundation of China (32260914; 32002387); National Marine Genetic Resource Center; the earmarked fund for CARS-49, and the Science and Technology Planning Project of Guangdong Province, China (2023B1212060047).

语种：英文

外文关键词：Crassostrea gigas; Crassostrea angulata; Allotriploid; Genetic diversity; Genetic structure

外文摘要：In bivalve aquaculture, tetraploids are commonly used to generate sterile triploids for commercial aquaculture. We obtained the allotriploid population (tetraploid C. gigas male x diploid C. angulata female) through the hybridization of tetraploid male C. gigas with diploid female C. angulata for the first time. However, the genetic diversity and genetic structure in successive mass selected generations of tetraploid C. gigas, diploid C. angulata and their allotriploid offspring are currently unknown. In this study, nine polymorphic microsatellite markers were used to evaluate their genetic diversity and genetic structure. The trend of reduced genetic diversity was recorded in successive generations of mass selected tetraploid C. gigas and diploid C. angulata. However, the diversity of the allotriploid population was significantly higher than that of the male parent population and the female parent population. This indicates that the hybridization technique of tetraploid C. gigas + diploid C. angulata to produce triploids is beneficial for increasing the genetic diversity of the hybrid triploid. Little genetic differentiation was found within tetraploid C. gigas and diploid C. angulata (except A3, the third generation of C. angulata), which is most likely caused by the insignificant genetic structure of the selected lines. The large genetic differences between tetraploid C. gigas and diploid C. angulata are caused by subspecies differences and ploidy difference. In addition, allotriploids were found to be more closely related to the female population, suggesting that this genetic relationship is more consistent with phenotypic traits. The findings of this study offer useful information for further genetic improvement of oyster polyploidy breeding.