文献类型：期刊文献

英文题名：Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology

作者：Wu, Hongliang[1,3];Li, Guoliang[2,4];Liu, Shucheng[4];Ji, Zhongyin[1,3];Zhang, Qiulong[1,3];Hu, Na[1,3];Suo, Yourui[1];You, Jinmao[1,2]

机构：[1]Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China;[2]Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China;[3]Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China;[4]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China

年份：2015

卷号：8

期号：3

起止页码：685

外文期刊名：FOOD ANALYTICAL METHODS

收录：SCI-EXPANDED(收录号：WOS:000349553900018)、、EI(收录号：20143600021736)、Scopus(收录号：2-s2.0-84923936603)、WOS

基金：This work was supported by The National Natural Science Foundation of China (No. 31301595 and No. 21275089), Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (GDPKLAPPS1401) and Ph.D. research start-up funds of Qufu Normal University (bsqd 2012017).

语种：英文

外文关键词：Biogenic amines; Fluorescence labeling; Ultrasound-assisted dispersive liquid-liquid microextraction; Foodstuff samples; Box-Behnken design

外文摘要：A simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) was developed for the determination of biogenic amines (BAs) in foodstuff samples by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). In this work, fluorescence probe 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) was applied to label BAs. What followed was the UA-DLLME procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling and DLLME efficiency. Under the optimal conditions, this method offered low limits of detection (LODs) of 1.1-7.8 ng/mL and limits of quantification (LOQs) of 3.5-26.1 ng/mL. Finally, the method was successfully used for the determination of trace BAs in real samples and exhibited powerful potential in the high-throughput sample screening.