文献类型：期刊文献

英文题名：Preparation and evaluation of chitosan/alginate porous microspheres/Bletilla striata polysaccharide composite hemostatic sponges

作者：Wang, Chao[1,2]; Luo, Wenfeng[1]; Li, Puwang[2]; Li, Sidong[3]; Yang, Ziming[2]; Hu, Zhang[3]; Liu, Yangyang[2]; Ao, Ningjian[1]

机构：[1] Institute of Biomedical Engineering, Jinan University, Guangzhou, 510632, China; [2] Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 524001, China; [3] School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China

年份：2017

卷号：174

起止页码：432

外文期刊名：Carbohydrate Polymers

收录：EI(收录号：20172803926633)、Scopus(收录号：2-s2.0-85021897963)

语种：英文

外文关键词：Alginate - Emulsification - Microspheres - Water absorption - Chitosan - Biocompatibility - Blood

外文摘要：We prepared a novel hemostatic material using chitosan (CS), alginate, and Bletilla striata, which showed good biocompatibility and hemostatic properties. Alginate-calcium porous microspheres (Alg) were prepared by emulsification/cross-linking methods, and Bletilla striata polysaccharide (Bsp) was extracted from the tubers by water extraction and alcohol precipitation. To improve the water absorption and accelerate the blood coagulation, Alg and Bsp were mixed with chitosan solution to form CS/Alg and CS/Alg/Bsp composite sponges by lyophilization. Based on SEM images, the microspheres exhibited good spherical shape, possessed many pores on the surface, and were well dispersed in the CS/Alg5 and CS/Alg10 composite sponges. The CS/Alg10 composite sponges showed higher water absorption and porosity. In addition, the cytotoxicity assay and hemostatic property of the CS/Alg and CS/Alg/Bsp composite sponges were tested by CCK-8 assay, in vitro blood clotting, red blood cell (RBC) adhesion, and rabbit ear artery bleeding. These ternary composite sponges could be used as potential novel hemostatic materials in surgical treatment. ? 2017 Elsevier Ltd