文献类型：期刊文献

英文题名：Characteristic study of Pb2+binding to extracellular polymeric substances from Staphylococcus epidermidis G257

作者：Chen, Yinyan[1];Fang, Zhijia[1];Li, Yongbin[1];Deng, Qi[1];Sun, Lijun[1];Liao, Jianmeng[2];Gooneratne, Ravi[3]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Coll Food Sci & Technol, Res Ctr Marine Food,Guangdong Prov Engn Technol,Gu, Zhanjiang 524088, Peoples R China;[2]Zhanjiang Inst Food & Drug Control, Zhanjiang 524022, Peoples R China;[3]Lincoln Univ, Dept Wine Food & Mol Biosci, Lincoln 7647, Canterbury, New Zealand

年份：2025

卷号：198

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：SCI-EXPANDED(收录号：WOS:001401185600001)、、EI(收录号：20250317695476)、Scopus(收录号：2-s2.0-85214862565)、WOS

基金：This work was supported by the National Natural Science Foundation of China (No. 32172215) , the Natural Science Foundation of Guangdong Province (No. 2024A1515011711) , The Special Project of Science and Technology Development of Zhanjiang (No. 2022A01014) .

语种：英文

外文关键词：Staphylococcus epidermidis; Lead; Adsorption; Extracellular polymeric substances (EPS)

外文摘要：As an important toxic pollutant, lead (Pb2+) seriously threatens the environment and human well-being. Microbes and microbial extracellular polymeric substances (EPS) are highly effective at removing heavy metals from solutions. Herein, a Pb2+-tolerant strain, Staphylococcus epidermidis G257 was isolated from Guangxi of China. The strain showed strong resistance to Pb2+ and great Pb2+ adsorbability. The biosorption processes of S. epidermidis G257 and its EPS were characterized using microscopical techniques, ICP-MS, zeta potential measurement, XRD, FTIR, 3D-EEM, and XPS spectroscopy. Results showed that S. epidermidis G257 were effective in adsorbing Pb2+, with a biosorption efficiency of 81%. The biosorption process of Pb2+ was described well by the pseudo-second-order model with an adsorption capacity of 53.52 mg/g. Microscopical analyses showed that Pb2+ was adsorbed on the S. epidermidis surface by forming EPS precipitates. The variations in zeta potential indicated an electrostatic attraction in the Pb2+ adsorption process. Moreover, FTIR, 3D-EEM and XPS analyses revealed that the functional groups participating in Pb2+ adsorption were primarily carbonyl, carboxyl and amino groups of proteins in the EPS, which greatly facilitated Pb2+ adsorption by forming EPS-metal complexes. Therefore, this study unveiled that S. epidermidis G257 can be utilized as a potential biosorbent for eliminating Pb2+ from polluted environment.