文献类型：期刊文献

英文题名：Differences in toxicity induced by the various polymer types of nanoplastics on HepG2 cells

作者：Ma, Lihua[1];Wu, Zijie[1];Lu, Zifan[2];Yan, Linhong[1];Dong, Xiaoling[1];Dai, Zhenqing[3,4];Sun, Ruikun[2];Hong, Pengzhi[1];Zhou, Chunxia[1];Li, Chengyong[2,3,4,5]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Engn Technol Res Ctr Seafood, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Coll Food Sci & Technol,Guangdong Prov Engn Lab Ma, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China;[4]Guangdong Ocean Univ, Guangdong Prov Key Lab Intelligent Equipment South, Zhanjiang 524088, Peoples R China;[5]Guangdong Ocean Univ, Guangdong Prov Observat & Res Stn Trop Ocean Envir, Zhanjiang 524088, Peoples R China

年份：2024

卷号：918

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：SCI-EXPANDED(收录号：WOS:001179519400001)、、EI(收录号：20240615520844)、Scopus(收录号：2-s2.0-85183945304)、WOS

基金：This work was supported by the Project of Enhancing School with Innovation of Guangdong Ocean University (2020ZDZX2029), Guangdong Basic and Applied Basic Research Foundation (2022B1515020091), Shenzhen Science and Technology Program (KCXFZ202002011011057, JCYJ20210324122606017, GJHZ20210705142000003) and Training Programs of Innovation and Entrepreneurship for Undergraduates (CXXL2022176). We would like to thank Analytical and Testing Center of Guangdong Ocean University for detection. The graphical abstract was drawn by Figdraw (www.figdraw.com).

语种：英文

外文关键词：Nanoplastics; HepG2 cells; Oxidative stress; Apoptosis

外文摘要：The problem of microplastics (MPs) contamination in food has gradually come to the fore. MPs can be transmitted through the food chain and accumulate within various organisms, ultimately posing a threat to human health. The concentration of nanoplastics (NPs) exposed to humans may be higher than that of MPs. For the first time, we studied the differences in toxicity, and potential toxic effects of different polymer types of NPs, namely, polyethylene terephthalate (PET), polyvinyl chloride (PVC), and polystyrene (PS) on HepG2 cells. In this study, PET -NPs, PVC -NPs, and PS -NPs, which had similar particle size, surface charge, and shape, were prepared using nanoprecipitation and emulsion polymerization. The results of the CCK-8 assay showed that the PET -NPs and PVC -NPs induced a decrease in cell viability in a concentration -dependent manner, and their lowest concentrations causing significant cytotoxicity were 100 and 150 mu g/mL, respectively. Moreover, the major cytotoxic effects of PET -NPs and PVC -NPs at high concentrations may be to induce an increase in intracellular ROS, which in turn induces cellular damage and other toxic effects. Notably, our study suggested that PET -NPs and PVC -NPs may induce apoptosis in HepG2 cells through the mitochondrial apoptotic pathway. However, no relevant cytotoxicity, oxidative damage, and apoptotic toxic effects were detected in HepG2 cells with exposure to PSNPs. Furthermore, the analysis of transcriptomics data suggested that PET -NPs and PVC -NPs could significantly inhibit the expression of DNA repair -related genes in the p53 signaling pathway. Compared to PS -NPs, the expression levels of lipid metabolism -related genes were down -regulated to a greater extent by PET -NPs and PVC -NPs. In conclusion, PET -NPs and PVC -NPs were able to induce higher cytotoxic effects than PS -NPs, in which the density and chemical structure of NPs of different polymer types may be the key factors causing the differences in toxicity.