文献类型：期刊文献

英文题名：VscO, a putative T3SS chaperone escort of Vibrio alginolyticus, contributes to virulence in fish and is a target for vaccine development

作者：Zhou, Zejun[1,2,3];Pang, Huanying[1,2,3];Ding, Yu[1,2,3];Cai, Jia[1,2,3];Huang, Yucong[1,2,3];Jian, Jichang[1,2,3];Wu, Zaohe[2,3,4]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[3]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China;[4]Zhongkai Univ Agr & Engn, Guangzhou, Guangdong, Peoples R China

年份：2013

卷号：35

期号：5

起止页码：1523

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000328006700019)、、WOS

基金：This work was supported by Natural Science Foundation of China (NSFC, No. 30972271), the 973 Program Earlier Research Project (No. 2011CB111601), National Science and Technology Supporting Project (No. 2012BAD17800) and Guangdong Education Department Program for Nursery Project (No. B12121) in the People's Republic of China. We thank all the people who have dedicated time to the experiments.

语种：英文

外文关键词：Vibrio alginolyticus; Type III secretion system; vscO mutant; Recombinant VscO; Protective immunity

外文摘要：Type III secretion system (T3SS) in Vibrio alginolyticus is essential for its pathogenesis. VscO's homologous proteins FliJ, InvI and YscO have been suggested to be putative chaperone escorts although its function in V. alginolyticus is unclear. To investigate the physiological role of VscO, a mutant strain of V. alginolyticus with an in-frame deletion of the vscO gene was constructed in the present study. One finding was that the mRNA expression levels of SycD, VopB and VopD proteins decreased in the Delta vscO mutant. In addition, the Delta vscO mutant showed an attenuated swarming ability and a ten-fold decrease in the virulence to fish. However, the Delta vscO mutant showed no difference in the biofilm formation and ECPase activity. Complementation of the mutant strain with the vscO gene could restore the phenotypes of the wild-type strain. Finally, the recombinant VscO protein caused a high antibody titer and an effective protection against lethal challenge with the wild-type strain V. alginolyticus. These results indicated that VscO protein has a specific role in the pathogenesis of V. alginolyticus and it may be a candidate antigen for development of a subunit vaccine against vibriosis. (C) 2013 Elsevier Ltd. All rights reserved.