文献类型：期刊文献

英文题名：Crustacean methyl farnesoate-binding protein is an insect juvenile hormone-binding protein homolog that inhibits molting

作者：Yang, Hao[1];Chen, Ting[2];Zhang, Xin[2];Zhou, Mingyu[3];Zhang, Lvping[2];Yan, Aifen[4];Chen, Weihao[5];Tan, Guiling[1];Liang, Jingxuan[2];Ren, Chunhua[2];Chen, Xiaoli[6];Li, Zhi[3];Ruan, Yao[2];Li, Jiaxi[4];Li, Hongmei[2];Luo, Peng[2];Wang, Yanhong[2];Jiang, Xiao[2];Yin, Jiayue[2];Ma, Bo[2];Zhu, Chunhua[6];Wu, Xugan[3];Zhang, Jiquan[1];Hu, Chaoqun[2]

机构：[1]Hebei Univ, Coll Life Sci, Lab Zool Systemat & Applicat Hebei Prov, Baoding, Peoples R China;[2]Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Lab Trop Marine Bioresources & Ecol,Guangdong Prov, Guangzhou, Peoples R China;[3]Shanghai Ocean Univ, Res Ctr Aquaculture Nutr & Environm Ecol, Minist Agr & Rural Affair, Shanghai, Peoples R China;[4]Foshan Univ, Sch Med, Foshan, Peoples R China;[5]Chinese Acad Med Sci, Fuwai Hosp, State Key Lab Cardiovasc Dis, Shenzhen, Peoples R China;[6]Guangdong Ocean Univ, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techno, Zhanjiang, Peoples R China

年份：2025

卷号：301

期号：7

外文期刊名：JOURNAL OF BIOLOGICAL CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:001521283800003)、、EI(收录号：20252618659189)、Scopus(收录号：2-s2.0-105008689052)、WOS

基金：This study was Supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (grant no.: XDB07302000) , Guangdong Province Project (grant no.: 2022-SPY-00-001) , the National Natural Science Foundation of China (grant no.: 32373121) , the Research on breeding technology of candidate species for Guangdong modern marine ranching (grant no.: 2024-MRB-00-001) , and the Innovation Team Project of High Level Local Universities from Shanghai Education Committee (grant no.: HJWK-2021-21) .

语种：英文

外文关键词：Biochemistry - Endocrinology - Genes - Hormones - Proteins

外文摘要：Methyl farnesoate (MF) serves as an essential regulator of key developmental processes in crustaceans, similar to juvenile hormones (JHs) in insects. However, it is susceptible to degradation in circulation. Despite the detection of proteins binding to MF in crustacean hemolymph for 3 decades, the precise genes encoding these proteins remain unclear. The present study identifies genes in crustaceans containing the juvenile hormone-binding protein (JHBP) domain. Among the 11 JHBPs found in the Pacific white shrimp (Litopenaeus vannamei), XP_027209752.1, which is primarily expressed in the hepatopancreas, emerges as the predominant form. This protein exhibits selective binding to MF rather than to JHs, leading to its designation as a methyl farnesoate-binding protein (MFBP) in crustaceans. Alterations in the amino acid sequence are predicted to induce structural changes that enhance the affinity of MFBP for MF. Endogenous MFBP inhibits molting, consistent with the function of MF. Furthermore, positive regulation of MFBP by MF has been observed in hepatopancreatic primary cells, with similar trends of hepatopancreatic MFBP mRNA and hemolymph MF levels during molting and ovarian development. This study identifies a novel MFBP in crustaceans that, despite being a homolog of insect JHBP, specifically binds MF to regulate molting in penaeid shrimp. These findings may advance our understanding of crustacean endocrine regulation and provide a molecular basis for improving their aquaculture techniques.