文献类型：期刊文献

中文题名：五味子木脂素超声提取工艺优化及其抗氧化和抗真菌的潜力

英文题名：Optimization of ultrasonic extraction technology of Shisandra lignans and its anti-oxidant and anti-fungi potentials

作者：李启思[1,2];王雅玲[1,2];邓玉华[1,2];廖建萌[1,2];叶林[1,2];吴莉莉[1,2];郑佳纯[1,2];罗杏燕[1,2];邓旗[1,2];孙力军[1,2]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东普通高等学校水产品深加工重点实验室,广东湛江524088;[2]湛江市食品药品检验所,广东湛江524037

年份：2021

卷号：33

期号：11

起止页码：2145

中文期刊名：浙江农业学报

外文期刊名：Acta Agriculturae Zhejiangensis

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：国家自然科学基金(31871898);广东省大学生创新创业训练计划(粤教高函〔2018〕42号);广东省现代农业产业技术体系创新团队(2019KJ149)。

语种：中文

中文关键词：五味子木脂素;液质联用;超声提取;响应面;抗氧化

外文关键词：Schisandra lignans;liquid chromatography-tandem mass spectrometry;ultrasonic extraction;response surface;antioxidation

中文摘要：为探究五味子木脂素的超声提取工艺,及其抗氧化和抗真菌的能力,建立起一种基于液相色谱-质谱联用法(LC-MS/MS)同步检测五味子中5种木脂素含量的方法,在此基础上,基于4项单因素试验和中心复合设计-响应面法优化五味子木脂素的超声提取工艺,并评价其抗氧化和抗水产干制品中优势真菌的能力。结果表明:适宜超声提取五味子木脂素的工艺参数为乙醇体积分数70%,液料比12 mL·g^(-1),提取2次,每次超声10 min。在此条件下,五味子木脂素提取量为(9.45±0.11)mg·g^(-1)。五味子木脂素提取液的总还原力,及对DPPH(1,1-二苯基-2-三硝基苯肼)、ABTS(2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸)自由基的清除能力分别是维生素C(V_(C))的2/5、1/2和1/3,对黄曲霉(Aspergillus flavus)、黑曲霉(Aspergillus niger)、橘青霉(Penicillium citrinum)、尖孢镰孢菌(Fusarium oxysporium)和厚垣镰孢菌(Fusarium chlamydosporum)的最低抑菌浓度(MIC)约为1.17 mg·mL^(-1),对哈茨木霉(Trichoderma harzianum)、扩展青霉(Penicillium expansum)和腐皮镰孢菌(Fusarium solani)的MIC约为2.34 mg·mL^(-1),说明其具有较强的抗氧化和抗水产干制品中优势真菌的能力。

外文摘要：To explore the suitable ultrasonic extraction technology of Schisandra lignans and its inhibitory effect against oxidant and dominant fungi in dried aquatic products,a synchronous detection methods for 5 kinds of lignans was established via LC-MS/MS(liquid chromatography-tandem mass spectrometry).On the basis,four single-factor tests,and the central composite response surface test were adopted to optimize the ultrasonic extraction parameters of Schisandra lignans,and the anti-oxidant and anti-fungi activities of Schisandra lignans were analyzed.It was shown that the suitable extraction parameters included ethanol volume fraction of 70%,liquid to material ratio of 12 mL·g^(-1),extraction times of twice,and ultrasound time of 10 min per time.Under this condition,the extraction amount of lignans was(9.45±0.11)mg·g^(-1).The total reducing power,and DPPH(1,1-diphenyl-2-picrylhydrazyl),ABTS[2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)]free radical scavenging ability of Schisandra lignans were 2/5,1/2 and 1/3 of vitamin C(V_(C)),respectively.The minimum inhibitory concentration(MIC)of Schisandra lignans against Aspergillus flavus,Aspergillus niger,Penicillium citrinum,Fusarium oxysporium,Fusarium chlamydosporum was about 1.17 mg·mL^(-1),and the MIC of Schisandra lignans against Trichoderma harzianum,Penicillium expansum,Fusarium solani was about 2.34 mg·mL^(-1),which indicated that the extracted Schisandra lignans had strong inhibitory effect against oxidant and dominant fungi in dried aquatic products.