文献类型：期刊文献

中文题名：新城疫病毒广东分离株F基因的克隆与序列分析

英文题名：Cloning and Sequence Analysis of F Gene of Newcastle disease virus Isolated from Guangdong Province

作者：刘铀[1];刘艳芬[2];陈绍红[1];马龙[3]

机构：[1]湛江海洋大学生化中心,广东湛江524088;[2]湛江海洋大学农学院,广东湛江524088;[3]湛江市畜牧局,广东湛江524038

年份：2005

卷号：26

期号：10

起止页码：60

中文期刊名：动物医学进展

外文期刊名：Progress In Veterinary Medicine

收录：CSTPCD、、CSCD_E2011_2012、CSCD

语种：中文

中文关键词：新城疫病毒;克隆;F基因;序列分析

外文关键词：Newcastle disease virus ; clone ; F gene ; sequence analysis

中文摘要：为研究新城疫病毒(NDV)基因变异情况,用RT-PCR扩增了9个广东分离株的F基因cDNA片段,并将其分别克隆到pGEM-Teasy载体中.序列分析结果表明,上述分离株F基因长度为1 662 bp,编码553个氨基酸,核苷酸和氨基酸序列同源性分别为95.1%～99.2%和96.0%～99.8%;但与LaSota、Roakin、F48E9及B1等常见疫苗株的氨基酸同源性仅为87.6%～92.2%.9个NDV分离株F蛋白裂解位点的氨基酸序列为112R-R-Q/R-K-R-F-I-G119,与平均致死鸡胚时间(MDT)、脑内接种指数(ICPI)等病毒致病力测定结果相符,均属于基因Ⅶ型NDV.

外文摘要：Strains of Newcasttle disease virus（NDV） isolated from Guangdong province, were used to study the genetic variation of NDV. The cDNA fragments of F gene of 9 NDV strains above were amplified by RT-PCR, then the amplified fragments were cloned into pGEM-Teasy vector and the recombinant plasmids were sequenced. The results showed that the F gene from all of the NDV isolates consisted of 1662 bp, coding for 553 amino acid. Neucleitide and amino acid sequence homologies were found to be 95. 1%- 99.2%and 96.0%-99.8% respectively, but the amino acid sequence homologies to other NDV strains such as LaSota,Roakin.F48E9 and B1 were only from 87.6% to 92.2%. The deduced amino acid sequence of the cleavage site of F protein of the 9 NDV isolates were ^112R-R-Q/R-K-R-F-I-G^119 , which was accordant with the results of mean death time of chicken embryos（MDT）and intracerebral pathogenicity index（ICPI） determination, and all of the 9 NDV isolates belonged to the genetype Ⅶ.