文献类型：期刊文献

英文题名：Carboxymethyl Cellulose and Carboxymethyl Chitosan-Based Composite Nanogel as a Stable Delivery Vehicle for Oyster Peptides: Characterization, Absorption and Transport Mechanism

作者：Chen, Zhongqin[1,2,3,4,5]; Zhang, Pei[1]; Zhou, Longjian[1]; Gao, Jialong[1,2,3,4,5,6]; Zheng, Huina[1,2,3,4,5,6]; Lin, Haisheng[1,2,3,4,5,6]; Zhu, Guoping[1,2,3,4,5,6]; Qin, Xiaoming[1,3,4,5,6]; Cao, Wenhong[1,2,3,4,5]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China; [2] Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, China; [3] National Research and Development Branch Center for Shellfish Processing [Zhanjiang], Zhanjiang, 524088, China; [4] Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang, 524088, China; [5] Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang, 524088, China; [6] Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230185785)

语种：英文

外文关键词：Cellulose - Chemical stability - Chitosan - Molecular biology - Nanostructured materials - Peptides

外文摘要：An oyster peptide (OPs)-loaded composite nanogel based on carboxymethyl cellulose and carboxymethyl chitosan (CMC@CMCS@OPs) was prepared, and the characterization, absorption and transport mechanism were further investigated. CMC@CMCS@OPs, a dense spherical microstructure with a diameter of ～64 nm, which enhanced the thermal and digestive stabilities of individual OPs and improved its retention rate of hypoglycemic activity in vitro. The swelling response and in-vitro release profiles showed that CMC@CMCS@OPs could help OPs achieve targeted and controlled release in the intestine. In addition, CMC@CMCS@OPs had no cytotoxicity on Caco-2 cells, and its apparent permeability coefficients increased 4.70-7.45 times compared with OPs, with the absorption rate increased by 129.38%. Moreover, the transcytosis of CMC@CMCS@OPs nanogel occurred primarily through the macropinocytosis pathway, endocytosis pathway and intestinal efflux transporter-mediated efflux. Altogether, these results suggested that CMC@CMCS@OPs nanogel could be as an effective OPs delivery device for enhancing its stability and absorption. ? 2023, The Authors. All rights reserved.