文献类型：期刊文献

英文题名：Antimicrobial peptide AMPNT-6 from Bacillus subtilis inhibits biofilm formation by Shewanella putrefaciens and disrupts its preformed biofilms on both abiotic and shrimp shell surfaces

作者：Deng, Qi[1]; Pu, Yuehua[2]; Sun, Lijun[1]; Wang, Yaling[1]; Liu, Yang[3]; Wang, Rundong[1]; Liao, Jianmeng[4]; Xu, Defeng[1]; Liu, Ying[1]; Ye, Riying[1]; Fang, Zhijia[1]; Gooneratne, Ravi[5]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, No. 1 Haida Road, Zhanjiang, Guangdong Province, 524088, China; [2] Bureau of Quality and technology Supervise of Zhanjiang City, Zhanjiang, 524022, China; [3] National Marine Products Quality Supervision & Inspection Center, Zhanjiang, 524000, China; [4] Zhanjiang Food and Drug Administration, Zhanjiang, 524000, China; [5] Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, Canterbury, 7647, New Zealand

年份：2017

卷号：102

起止页码：8

外文期刊名：Food Research International

收录：EI(收录号：20174004240530)、Scopus(收录号：2-s2.0-85030311722)

语种：英文

外文关键词：Bacteriology - Shellfish - Bacteria - Losses - Steel sheet - Adhesion - Scanning electron microscopy - Spoilage - Strain - Stainless steel - Polystyrenes

外文摘要：Shewanella putrefaciens biofilm formation is of great concern for the shrimp industry because it adheres easily to food and food-contact surfaces and is a source of persistent and unseen contamination that causes shrimp spoilage and economic losses to the shrimp industry. Different concentrations of an antimicrobial lipopeptide, the fermentation product of Bacillus subtilis, AMPNT-6, were tested for the ability to reduce adhesion and disrupt S. putrefaciens preformed biofilms on two different contact surfaces (shrimp shell, stainless steel sheet). AMPNT-6 displayed a marked dose- and time-dependent anti-adhesive effect > biofilm removal. 3MIC AMPNT-6 was able both to remove biofilm and prevent bacteria from forming biofilm in a 96-well polystyrene microplate used as the model surface. 2MIC AMPNT-6 prevented bacteria from adhering to the microplate surface to form biofilm for 3 h and removed already existing biofilm within 24 h. Secretion of extracellular polymeric substances incubated in LB broth for 24 h by S. putrefaciens was minimal at 3 × MIC AMPNT-6. Scanning electron microscopy showed that damage to S. putrefaciens bacteria by AMPNT-6 possibly contributed to the non-adherence to the surfaces. Disruption of the mature biofilm structure by AMPNT-6 contributed to biofilm removal. It is concluded that AMPNT-6 can be used effectively to prevent attachment and also detach S. putrefaciens biofilms from shrimp shells, stainless steel sheets and polystyrene surfaces. ? 2017 Elsevier Ltd