文献类型：期刊文献

英文题名：Sponges of Carboxymethyl Chitosan Grafted with Collagen Peptides for Wound Healing

作者：Cheng, Yu[1];Hu, Zhang[1];Zhao, Yuntao[2];Zou, Zuhao[1];Lu, Sitong[1];Zhang, Bijun[1];Li, Sidong[1]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China

年份：2019

卷号：20

期号：16

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：SCI-EXPANDED(收录号：WOS:000484411100047)、、Scopus(收录号：2-s2.0-85071281903)、WOS

基金：We gratefully acknowledge the financial support of the Natural Science Foundation of Guangdong Province of China (2016A030308009), Project of Science and Technology Plan of Guangdong Province (2017A010103023), the Project of Enhancing School with Innovation of Guangdong Ocean University (2017KTSCX090), and the Graduate Education Innovation Program of Guangdong Ocean University (201926).

语种：英文

外文关键词：carboxymethyl chitosan; collagen peptides; burns; wound healing

外文摘要：Burns are physically debilitating and potentially fatal injuries. Two marine biomaterials, carboxymethyl chitosan (CMC) and collagen peptides (COP), have emerged as promising burn dressings. In this paper, sponges of carboxymethyl chitosan grafted with collagen peptide (CMC-COP) were prepared by covalent coupling and freeze drying. Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy were then used to characterize the prepared sponges. To evaluate the wound healing activity of the CMC-COP sponges, in vitro tests including cell viability scratch wound healing and scald wound healing experiments were performed in rabbits. Appearance studies revealed the porous nature of sponges and FTIR spectroscopy demonstrated the successful incorporation of COP into CMC. The in vitro scratch assay showed that treatment with CMC-COP sponges (at 100 mu g/mL) had significant effects on scratch closure. For burn wounds treated with CMC-COP, regeneration of the epidermis and collagen fiber deposition was observed on day 7, with complete healing of the epidermis and wound on days 14 and 21, respectively. Based on the pathological examination by hematoxylin and eosinstaining, the CMC-COP group demonstrated pronounced wound healing efficiencies. These results confirmed that the CMC-COP treatment enhanced cell migration and promoted skin regeneration, thereby highlighting the potential application of these sponges in burn care.