文献类型：期刊文献

英文题名：Metabolomics reveals the reproductive abnormality in female zebrafish exposed to environmentally relevant levels of climbazole

作者：Zou, Ting[1];Liang, Yan-Qiu[2];Liao, Xiaoliang[1];Chen, Xiao-Fan[1];Wang, Tao[3];Song, Yuanyuan[3];Lin, Zhi-Cheng[1];Qi, Zenghua[1];Chen, Zhi-Feng[1,4];Cai, Zongwei[1,3]

机构：[1]Guangdong Univ Technol, Inst Environm Hlth & Pollut Control, Sch Environm Sci & Engn, Guangdong Key Lab Environm Catalysis & Hlth Risk, Guangzhou 510006, Peoples R China;[2]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[3]Hong Kong Baptist Univ, Dept Chem, State Key Lab Environm & Biol Anal, Hong Kong, Peoples R China;[4]South China Normal Univ, Guangdong Prov Key Lab Chem Pollut & Environm Saf, Guangzhou 510006, Peoples R China

年份：2021

卷号：275

外文期刊名：ENVIRONMENTAL POLLUTION

收录：SCI-EXPANDED(收录号：WOS:000625380600062)、、EI(收录号：20210709912204)、Scopus(收录号：2-s2.0-85100635173)、WOS

基金：We would be grateful for the financial support from the National Natural Science Foundation of China (21507163 and 41907346), Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety (2019B030301008), and National Key Research and Development Project (2019YFC1804604).

语种：英文

外文关键词：Azole fungicide; Zebrafish; Metabolomics; Reproductive toxicity; Oxidative stress

外文摘要：Climbazole (CBZ) ubiquitously detected in the aquatic environment may disrupt fish reproductive function. Thus far, the previous study has focused on its transcriptional impact of steroidogenesis-related genes on zebrafish, but the underlying toxic mechanism still needs further investigation at the metabolic level. In this study, adult zebrafish were chronically exposed to CBZ at concentrations of 0.1 (corresponding to the real concentration in surface water), 10, and 1000 mu mg/L and evaluated for reproductive function by egg production, with subsequent ovarian tissue samples taken for histology, metabolomics, and other biochemical analysis. After 28 days' exposure, fecundity was significantly decreased in all exposure groups, with the inhibition of oocytes in varying developmental stages to a certain degree. The decrease in retinoic acid and sex hormones, down-regulated genes important in steroidogenesis, and increase in oxidized/reduced glutathione ratio and occurrence of apoptotic cells were observed in zebrafish ovaries following exposure to CBZ even at environmentally realistic concentrations, suggesting that alternations in steroidogenesis and oxidative stress can play significant roles in CBZ-triggered reproductive toxicity. Besides, mass spectrometry imaging analysis validated the results from metabolomics analysis. Our findings provide novel perspectives for unveiling the mechanism of reproductive dysfunction by CBZ and highlight its risk to fish reproduction. (C) 2021 Elsevier Ltd. All rights reserved.