文献类型：期刊文献

英文题名：Walnut protein hydrolysates, rich with peptide fragments of WSREEQEREE and ADIYTEEAGR ameliorate UV-induced photoaging through inhibition of the NF-κB/MMP-1 signaling pathway in female rats

作者：Xu, Defeng[1];Wang, Weiqiong[2];Liao, Jianmeng[1];Liao, Lan[3];Li, Caihong[2];Zhao, Mouming[4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Guangdong, Peoples R China;[2]Guangdong Med Univ, Sch Basic Med, Inst Biochem & Mol Biol, Dongguan 523808, Guangdong, Peoples R China;[3]Foshan Univ, Coll Food Sci & Technol, Dept Food Sci, Foshan 528000, Guangdong, Peoples R China;[4]South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Peoples R China

年份：2020

卷号：11

期号：12

起止页码：10601

外文期刊名：FOOD & FUNCTION

收录：SCI-EXPANDED(收录号：WOS:000599960700027)、、EI(收录号：20205209680099)、Scopus(收录号：2-s2.0-85098463975)、WOS

基金：The authors gratefully acknowledge the State Key Research and Development Plan (No. 2017YFD0400200) and the Fundamental Research Funds for the Central Universities (No. 20152M062) for the financial support.

语种：英文

外文关键词：Chemical activation - Hydrolysis - Peptides - Signaling

外文摘要：Skin photoaging is a complicated pathological process, and the imbalance of inflammatory regulation is associated highly with photoaging progression. Previously, prepared walnut protein hydrolysates (WPH), rich with peptide fragments of WSREEQEREE and ADIYTEEAGR demonstrated desirable photoprotection. However, it remains unclear if the photoprotection is mediated by the targeted inhibition of the NF-kappa B signaling pathway. Herein, we examined the regulation of WPH on inflammatory cytokine expression, and elucidated the modulation of the NF-kappa B/MMP-1 signaling pathway by WPH in a photoaging SD rat model. WPH significantly reduced the expression level of inflammatory cytokines IL-1 beta and IL-6, but significantly increased the level of IL-2 (all P < 0.05). Furthermore, WPH dramatically inhibited the activation of the NF-kappa B signaling pathway by mitigating the phosphorylation of I kappa B and p-65 proteins in a dose-dependent manner. The histopathological results indicated that WPH predominately attenuated epidermal hyperplasia, reduced the inflammatory filtration, and promoted collagen deposition in the photoaging skin tissue. Furthermore, WPH significantly stimulated the expression of TGF-beta and procollagen type I, and inhibited the MMP-1 activities (all P < 0.05). Overall, the underlying mechanism of WPH ameliorating skin photoaging may be attributed to the synergistic modulation via reversing the inflammatory imbalance, suppressing the activation of the NF-kappa B signal pathway, stimulating procollagen type I synthesis, and inhibiting MMP-1 activities. According to these results, it can be concluded that WPH has the potential as an anti-photoaging agent in functional foods.