文献类型：期刊文献

英文题名：Molecular and functional characterization of interferon regulatory factor 1 (IRF1) in amphibian Xenopus tropicalis

作者：Gan, Zhen[1,2,3,4,5,6];Cheng, Jun[1,3,4,5];Hou, Jing[1,2,3,4,5,6];Xia, Liqun[1,3,4,5];Lu, Yishan[1,3,4,5];Nie, Pin[2]

机构：[1]Guangdong Ocean Univ, Shenzhen Inst, 3 Binhai 2nd Rd, Shenzhen 518120, Guangdong, Peoples R China;[2]Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China;[3]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524025, Peoples R China;[4]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen 518120, Peoples R China;[5]Shenzhen Publ Serv Platform Evaluat Marine Econ A, Shenzhen 518120, Peoples R China;[6]Shenzhen Dapeng New Dist Sci & Technol Innovat Se, Shenzhen 518120, Peoples R China

年份：2021

卷号：167

起止页码：719

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:000606683200065)、、EI(收录号：20241015685383)、Scopus(收录号：2-s2.0-85097459658)、WOS

基金：We thank all the laboratory members for their critical reviews and comments on this manuscript. This work was financially supported by a project funded by China Postdoctoral Science Foundation (2020M682529), and by a grant from China Agricultural Research System (CARS-46).

语种：英文

外文关键词：Amphibian; Xenopus tropicalis; Interferon regulatory factor; IRF1; Interferon-stimulated response element

外文摘要：Interferon regulatory factor 1 (IRF1) is an important regulator in controlling the transcription of type I interferon genes, and its functions have been well-characterized in mammals, birds and fish. However, little information is available regarding the function of amphibian IRF1. In this study, an IRF1 gene homolog named as Xt-IRF1 was identified in the Western clawed frog (Xenopus tropicalis), an amphibian model specie widely used for comparative immunology research. Xt-IRF1 and IRF1 in other vertebrates possess similar genomic structure and flanking genes, and were grouped together to form a separate clade in phylogenetic tree. In addition, Xt-IRF1 gene was constitutively expressed in all tissues examined, with the highest expression level observed in spleen, and was inducible after poly(I:C) stimulation. Importantly, the expression of Xt-IRF1 was markedly induced by recombinant type I interferon, and Xt-IRF1 induced a strong activation of both IFN beta and ISRE promoters. The present study opens the door to investigate the roles of IRF1 in amphibians, and thus contributes to a better understanding of the functional evolution of IRFs in lower tetrapods. (C) 2020 Published by Elsevier B.V.