文献类型：期刊文献

英文题名：Response of pearl oyster Pinctada fucata martensii to allograft-induced stress from lipid metabolism

作者：He, Chengzhang[1];Hao, Ruijuan[1];Deng, Yuewen[1,2];Yang, Chuangye[1,2];Du, Xiaodong[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang 524088, Peoples R China

年份：2020

卷号：98

起止页码：1001

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000516880900105)、、WOS

基金：This work was supported by "Innovation Team Project" (Grant number: 2017KCXTD016) from the Department of Education of Guangdong Province, the Science and Technology Department of Guangdong Province (Grant number: 2018A030310666, 2017A030307024 and 2017A030303076), and the National Natural Science Foundation of China (Grant numbers: 31672626).

语种：英文

外文关键词：Pinctada fucata martensii; Lipid metabolism; Fatty acids; Transcriptome

外文摘要：The pearl oyster, Pinctada fucata martensii, produces high-quality pearls. During pearl production, excess immune and inflammatory response after transplantation will lead to nucleus rejection, pearl sac formation failure, and death of the host pearl oyster. The hemocyte transcriptome and fatty acid (FA) contents in the adductor muscle before and after transplantation were analyzed to investigate the response of pearl oyster P. f. martensii to allograft-induced stress from lipid metabolism. The hemocyte transcriptome analysis detected 193 lipid metabolism-related genes, such as the elongation of very long-chain FA protein 5, acyl-CoA 6-desaturase, cytochrome P450, phospholipase A2, glycerol-3-phosphate O-acyltransferase, and prostaglandin-H2 D-isomerase. Pathway enrichment analyses revealed that these genes were mainly involved in the "biosynthesis of unsaturated FM," "FA biosynthesis," "ARA metabolism," and "glycerolipid metabolism." An analysis of FA contents in the adductor muscle indicated no significant difference in the contents of lauric acid, myristic acid, pentadecanoic acid, palmitic acid, palmitoleic acid, heptadecanoic acid, stearic acid, oleic acid, vaccenic acid, linoleic acid, arachidic acid, alpha-linolenic acid, eicosadienoic acid, docosadienoic acid, and 11,14,17-eicosatrienoic acid. However, ARA, DHA, and EPA in the adductor muscle after transplantation were significantly greater than those processed without grafting surgery. These results suggest that pearl oysters require more polyunsaturated FAs (PUFAs) to regulate their inflammatory and immune response after transplantation. However, their ability to biosynthesize unsaturated FM is limited. Given these results, the addition of PUFA-containing diets or selection of a line with strong ability to biosynthesize unsaturated FM may be valuable for pearl oyster recovery after transplantation.