文献类型：期刊文献

英文题名：Cellular properties of the fermented microalgae pavlova lutheri and its isolated active peptide in osteoblastic differentiation of MG-63 cells

作者：Qian Z.-J.; Ryu B.; Kang K.-H.; Heo S.-J.; Kang D.-H.; Bae S.Y.; Park S.-J.; Kim J.-I.; Kim Y.-M.; Kim Y.-T.; Jung W.-K.

机构：[1]Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China;[2]School of Pharmacy, University of Queensland, Brisbane, 4072, QLD, Australia;[3]Marine Bioprocess Research Center, Pukyong National University, Busan, 48513, South Korea;[4]Jeju International Marine Science Center for Research and Education, Korea Institute of Ocean Science and Technology, Jeju, 63349, South Korea;[5]Department of Naval Architecture, Marine Systems Engineering, South Korea;[6]Department of Chemistry, Food Science and Nutrition, Food Science and Technology, Pukyong National University, Busan, 48513, South Korea;[7]Food Science and Nutrition, Food Science and Technology, Pukyong National University, Busan, 48513, South Korea;[8]Food Science and Technology, Pukyong National University, Busan, 48513, South Korea;[9]Department of Food Science and Biotechnology, Kunsan National University, Kunsan, North Jeolla, 573-701, South Korea;[10]Department of Biomedical Engineering, Center for Marine-Integrated Biomedical Technology (BK21 Plus), Marine-Integrated Bionics Research Center, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan, 48513, South Korea

年份：2018

卷号：17

期号：1

起止页码：2044

外文期刊名：Molecular Medicine Reports

收录：Scopus(收录号：2-s2.0-85034991198)

语种：英文

外文关键词：Fermented microalgae Pavlova lutheri; Osteoblastic differentiation; Peptide

外文摘要：Fermented microalgae Pavlova lutheri (P. lutheri), the product of Hansenula polymorpha fermentation, exhibited an increase in alkaline phosphatase (ALP) activity in MG-63 osteoblastic cells when compared to that of non-fermented P. lutheri. Fractionation of the fermented P. lutheri resulted in identification of the active peptide [peptide of P. lutheri fermentation (PPLF)] with the sequence of EPQWFL. PPLF significantly increased ALP release from MG-63 cells and mineralization in a dose-dependent manner. In addition, the intracellular levels of ALP and osteocalcin (OCN) proteins were augmented by PPLF treatment. To identify the molecular mechanism underlying the effect of PPLF on osteoblastic differentiation, the phosphorylation levels of the mitogen-activated protein kinases, p38, extracellular signal-regulated kinases 1/2 and Jun, and nuclear factor (NF)-κB were determined following PPLF treatment and the differences in expression were analyzed using p38 and NF-κB selective inhibitors. These results concluded that PPLF from fermented P. lutheri induced osteoblastic differentiation by increasing ALP and OCN release in MG-63 cells via the p38/p65 signaling pathway, indicating that PPLF supplement may be effective for therapeutic application in the field of bone health.