文献类型：期刊文献

英文题名：Potential Use of Chromolaena odorata Linn. Flavonoids against Escherichia coli Induced Diarrhoea in Mice

作者：Shi, Chao-Qun[1];Lin, Hong-Ying[1];Zheng, Jin-Qing[1];Yang, Fan[1];Lartey, Kwame Ayisi[1];Kang, Dan-Ju[1];Wang, Hwa-Chian Robert[2];Gooneratne, Ravi[3];Chen, Jin-Jun[1]

机构：[1]Guangdong Ocean Univ, Coll Agr Sci, Dept Vet Med, Zhanjiang 524088, Guangdong, Peoples R China;[2]Univ Tennessee, Coll Vet Med, Dept Biomed & Diagnost Sci, Knoxville, TN 37996 USA;[3]Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, Lincoln 7647, New Zealand

年份：2021

卷号：53

期号：6

起止页码：2269

外文期刊名：PAKISTAN JOURNAL OF ZOOLOGY

收录：SCI-EXPANDED(收录号：WOS:000716994900031)、、WOS

基金：The study was supported by the Special Competitive Allocation Project (No. 2013A3027) of the Guangdong Province Zhanjiang Programs for Financial Foundation of Science and Technology and Guangdong Postgraduate Education Innovation Project (2020SFJD001) .

语种：英文

外文关键词：Chromolaena odorata Linn.; Bacteria; Immunoglobulin; TGF-beta 1; Mouse

外文摘要：Flavonoids are important chemotaxonomic markers for the genus Chromolaena. Chromolaena odorata Linn. (CO) is used in folklore medicines to treat gastrointestinal diseases such as diarrhoea. This study examined the feasibility of total flavonoids from C. odorata (TFCO) as an antibacterial agent in vitro, and the mechanism of action of TFCO in combating Escherichia coli-induced diarrhoea in mice. MBC and MIC values of 0.25 and 0.125 g TFCO/ml showed it was effective as an antibacterial agent against E. coli (CMCC 44752) in vitro. Ninety Kunming mice were randomly divided into five groups of 18 mice per group (1 male: 1 female). The groups were control (saline), negative control (E. coli + 0 mg/kg TFCO), low dose (E. coli + 10 mg/kg TFCO), medium dose (E. coli + 40 mg/kg TFCO), and high dose (E. coli + 160 mg/kg). TFCO was administered by gavage 3 h post intraperitoneal (i.p) E. coli injection. Serum IgM, IgA, IgG, TGF-beta 1 mRNA expression in duodenal villi, and histopathology of duodenum were also studied. TFCO significantly increased the IgA and IgG concentration (P<0.05), reversed the intestinal mucosal damage caused by E. coli, and increased the relative expression of TGF-beta 1 mRNA. Therefore, TFCO has potential to improve immune function against E. coli infection, restored the intestinal structure and cured the E. coli-induced diarrhoea in mice.