文献类型：期刊文献

英文题名：Inflammation and apoptosis pathways mediated the stress response of Litopenaeus vannamei to acute cold and air exposure during waterless live transportation: Based on ultrastructure and transcriptome

作者：Zheng, Xiaoxian[1];Xu, Defeng[1,2];Sun, Lijun[1];Qin, Xiaoming[1];Zhang, Ying[3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Engn Technol Res Ctr Marine Food, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[2]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China;[3]Guangdong Ocean Univ, Sch Math & Comp Sci, Zhanjiang 524088, Peoples R China

年份：2022

卷号：131

起止页码：391

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000882960800002)、、WOS

基金：This study was financially supported by the National Key Research and Development Program of China (grant number: 2019YFD0901601) , the National Natural Science Foundation from China (grant number: 31772048) , and Postgraduate Education Innovation Project of Guang- dong Ocean University (grant number: 202261) .

语种：英文

外文关键词：L; vannamei; Waterless live transportation; Oxidative stress; Apoptosis; Inflammation

外文摘要：the combination of acute cold (AC) and waterless duration (WD) constitutes the major environmental stress and induces the damage or even mortality to shrimp L. vannamei during live transport, whereas the responding mechanism to AC symbolscript WD at molecular level remains unknown. The present study aims to clarify the responding mechanism of L. vannamei to AC symbolscript WD stress by ultrastructural observation and transcriptomic analysis on hepatopancreas tissue. The results showed that the dramatical oxidative stress induced by AC symbolscript WD significantly mediated the alteration of amino acids and energy metabolism. Furthermore, KEGG pathway enrichment anal-ysis revealed that the genes including DDO, GOT1, IDH1 and BBOX1 involved in energy metabolism and were significantly down-regulated, while some apoptosis-and inflammation-related genes such as DRONC, AP-1, and COX-2 were significantly up-regulated under AC symbolscript WD stress in comparison with those at normal control (all p < 0.05 or 0.01). These findings suggested that metabolic processes mediate the stress-induced damages of L. vannamei during waterless transport. Moreover, the significant overexpression of apoptosis-and inflammation -related proteins, and levels of inflammation cytokines in serum of shrimps strongly demonstrated the implication of inflammation and apoptosis pathways in stress-induced ultrastructural damage. These findings deepen our understanding into the response mechanisms of L. vannamei to AC symbolscript WD stress and provide the potential controlling biomarkers for transportation management.