文献类型：期刊文献

英文题名：Single-cell transcriptome landscape of zebrafish liver reveals hepatocytes and immune cell interactions in understanding nonalcoholic fatty liver disease

作者：Huang, Yingyi[1,2];Liu, Xiang[1,2];Wang, Hong-Yan[1,2];Chen, Jian-Yang[3,4];Zhang, Xianghui[3,4];Li, Yubang[1,2];Lu, Yifang[3,4];Dong, Zhongdian[5];Liu, Kaiqiang[1,2];Wang, Zhongduo[5];Wang, Qian[1,2,3];Fan, Guangyi[3,4,7];Zou, Jun[8];Liu, Shanshan[6,7];Shao, Changwei[1,2]

机构：[1]Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Natl Key Lab Mariculture Biobreeding & Sustainable, Qingdao 266072, Shandong, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266072, Shandong, Peoples R China;[3]BGI Res, Qingdao 266555, Shandong, Peoples R China;[4]BGI Res, Qingdao Key Lab Marine Genom, Qingdao 266555, Shandong, Peoples R China;[5]Guangdong Ocean Univ, Guangdong Higher Educ Inst, Fisheries Coll, Key Lab Aquaculture South China Sea Aquat Econ Ani, Zhanjiang 524088, Guangdong, Peoples R China;[6]MGI Tech, Shenzhen 518083, Peoples R China;[7]BGI Res, Shenzhen 518083, Peoples R China;[8]Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China

年份：2024

卷号：146

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001186711700001)、、Scopus(收录号：2-s2.0-85184905133)、WOS

基金：This study was supported by the National Key R & D Program of China (2022YFD2400100) ; the AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology (2017ASTCP-ES06) ; the Taishan Scholars Program (NO. tstp20221149 to C.S.) ; the National Ten-Thousands Talents Special Support Program to C.S.; the Central Public-interest Scientific Institution Basal Research Fund, CAFS (No.2020TD19) ; and the Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS (No. 20603022022002) .

语种：英文

外文摘要：Nonalcoholic fatty liver disease (NAFLD) is becoming the most common chronic liver disease in the world. Immunity is the major contributing factor in NAFLD; however, the interaction of immune cells and hepatocytes in disease progression has not been fully elucidated. As a popular species for studying NAFLD, zebrafish, whose liver is a complex immune system mediated by immune cells and non -immune cells in maintaining immune tolerance and homeostasis. Understanding the cellular composition and immune environment of zebrafish liver is of great significance for its application in NAFLD. Here, we established a liver atlas that consists of 10 cell types using single -cell RNA sequencing (scRNA-seq). By examining the heterogeneity of hepatocytes and analyzing the expression of NAFLD-associated genes in the specific cluster, we provide a potential target cell model to study NAFLD. Additionally, our analysis identified two subtypes of distinct resident macrophages with inflammatory and non -inflammatory functions and characterized the successive stepwise development of T cell subclusters in the liver. Importantly, we uncovered the possible regulation of macrophages and T cells on target cells of fatty liver by analyzing the cellular interaction between hepatocytes and immune cells. Our data provide valuable information for an in-depth study of immune cells targeting hepatocytes to regulate the immune balance in NAFLD.