文献类型：期刊文献

中文题名：牡蛎酶解产物的组成特点及其体外免疫活性

英文题名：Composition characteristics of oyster enzymatic hydrolysate and its immune activity in vitro

作者：李婉[1];曹文红[1,2];章超桦[1,2];秦小明[1,2];高加龙[1,2];郑慧娜[1,2]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东省水产品加工与安全重点实验室水产品深加工广东普通高等学校重点实验室国家贝类加工技术研发分中心(湛江)南海生物资源开发与利用协同创新中心,广东湛江524088

年份：2017

卷号：38

期号：16

起止页码：35

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、北大核心2014、CSCD_E2017_2018、北大核心、CSCD

基金：国家现代农业产业技术体系(CARS-48-07B)

语种：中文

中文关键词：牡蛎解产物;脾淋巴细胞;腹腔巨噬细胞;免疫调节

外文关键词：hydrolysates of oysters; spleen lymphocyte; peritoneal macrophages; immunoregulation;

中文摘要：香港牡蛎是我国南方高产低值的一种海洋贝类。为了提高其附加值,本研究以香港牡蛎为原料,通过酶法水解结合细胞实验对酶解产物及其超滤组分进行组成特点分析和免疫活性评价。结果表明:牡蛎酶解产物及各超滤组分分子量分布主要集中在<2.5 k Da范围内,含量达53.00%~85.00%,且氨基酸组成齐全;牡蛎酶解产物可促进脾淋巴细胞和腹腔巨噬细胞的免疫功能,各超滤组分(>10、10~5、5~2.5和<2.5 k Da)在浓度为0.25~4.0 mg/m L范围内,其体外免疫活性强弱具有一定的浓度相关性,且<2.5 k Da组分和5~2.5 k Da组分的免疫功能优于其他组分。牡蛎酶解产物各超滤组分中<2.5 k Da组分和5~2.5 k Da组分可作为进一步研究的对象。

外文摘要：Hongkong oyster is a king of marine shellfish in southern China with high yield and low value.In order to increase its added value,this study took Hongkong oyster as raw materials,through enzymatic hydrolysis combined with cell experiment of hydrolysates and its ultrafiltration fractions analyzed the composition characteristics and immune activity evaluation.The results indicated that the oyster hydrolysates and its ultrafiltration fractions molecular weight distribution were mainly concentrated in the 2.5 k Da,and the content was 53.00% -85.00%,and the complete amino acid composition.The oyster hydrolysates in vitro can promote the immune function of mice spleen lymphocytes and peritoneal macrophages.Further study on the immune activity of oyster hydrolysates,in the concentration range of 0.25 - 4.0 mg/m L,in vitro assays of cellular immune captivity of different ultrafiltration products（ 10,10 - 5,5 - 2.5 k Da and 2.5 k Da） hasa certain concentration correlation,and 2.5 k Da and5-2.5 k Da components have significant immunomodulatory effects. 2.5 k Da and 5 - 2.5 k Da afractions of each oyster hydrolysates ultrafiltration components may be used as an object for further study.