文献类型：期刊文献

英文题名：Molecular mechanisms of hypoxic preconditioning in the pearl oyster Pinctada fucata martensii revealed by DNA methylation profiling and transcriptome sequencing

作者：Yang, Chuangye[1];Huang, Jing[1];Luo, Junpeng[1];Wu, Qian[1];Hao, Ruijuan[2];Liao, Yongshan[3,4,5];Wang, Qingheng[1,3,4,5];Deng, Yuewen[1,3,4,5]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhanjiang, Dev & Res Ctr Biol Marine Resources, Zhanjiang 524006, Peoples R China;[3]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang, Peoples R China;[4]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[5]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China

年份：2026

卷号：613

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001621412700001)、、WOS

基金：innovation technology for Guangdong modern marine ranching (Grant no. 2024-MRI-001-03) , the Department of Education of Guangdong Province (Grant no. 2021KCXTD026) , and the program for scientific research start-up funds of Guangdong Ocean University (Grant no. 060302022304) . We thank TopEdit ( www.topeditsci.com ) for linguistic assistance during the preparation of this manuscript.

语种：英文

外文关键词：Pinctada fucata martensii; Hypoxic preconditioning; Hypoxia tolerance; DNA methylation; Transcriptome

外文摘要：Hypoxia poses a significant threat to aquaculture. Enhancing hypoxia tolerance in aquaculture animals is therefore critically important. Here, we examined the protective effects of HPD on the pearl oyster Pinctada fucata martensii and investigated the underlying mechanisms using transcriptome sequencing and DNA methylation profiling. The experimental groups comprised HPG (eight cycles at 2.0 mg/L for 24 h followed by 48 h of recovery) and CG (without HPD), both of which were exposed to a 15-d hypoxic challenge (1.5 mg/L). The survival rate was 36.96 % higher in the HPG than in the CG after 15 d of hypoxic stress. Physiological analyses revealed enhanced activities of antioxidant enzymes, digestive enzymes, and energy metabolism-related enzymes and lower malondialdehyde levels in HPG compared with CG. Transcriptomic profiling revealed that 1253 DEGs were enriched in pathways such as glutathione metabolism, lipid metabolism, and serotonergic synapse. DNA methylation analysis revealed that CG-type methylation was predominant in pearl oysters, with 21,430 CG-type DMRs identified between HPG and CG. DMR-associated genes were significantly enriched in 55 KEGG pathways, including the MAPK signaling pathway, protein processing in the endoplasmic reticulum, and glycolysis/gluconeogenesis. The 217 genes shared between CG-type DMR-associated genes and DEGs were enriched in tryptophan metabolism, pyruvate metabolism, glycolysis/gluconeogenesis, and the synaptic vesicle cycle. Our findings suggest that hypoxic preconditioning enhances hypoxia tolerance by reinforcing antioxidant defenses, shifting energy metabolism from glycolysis to lipid and amino acid utilization, regulating apoptosisrelated pathways, and preserving neural function, thereby enhancing the resilience of oysters to hypoxic stress in aquaculture systems.