文献类型：期刊文献

英文题名：Establishing LMBV infection resistance models in largemouth bass: Exploration and confirmation of pathways and genes relevant to disease resistance in breeding programs

作者：Li, Shen[1];Meng, Bangsong[1,2];Ouyang, Wenxuan[1,2];Wang, Liqun[3];Chen, Jinpeng[1];He, Xin[1];Qin, Qiwei[1,2];Yang, Min[1,2]

机构：[1]South China Agr Univ, Coll Marine Sci, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China;[2]Nansha South China Agr Univ, Fishery Res Inst, Guangzhou 511464, Peoples R China;[3]Guangdong Ocean Univ, Coll Fishery, Guangdong Key Lab Aquat Anim Dis Control & Hlth Cu, Southern Marine Sci & Engn Guangdong Lab Zhanjiang, Zhanjiang 524088, Peoples R China

年份：2026

卷号：610

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001527192700001)、、WOS

基金：The work was supported by grants from the Seed Industry Vitalizatio Project in Rural Vitalization Strategy of Guangdong, China 2024SPY00019; Special Science Found of Nansha-South China Agricultural University Fishery Research Institute, Guangzhou (NSYYKY202304); Guangzhou Rural Science and Technology Commissioner Project (2023E04J0121) and the National Natural Science Foundation of China (42306100).

语种：英文

外文关键词：Largemouth bass; Largemouth bass virus; RNA-seq; Disease resistance breeding; Candidate pathways and genes

外文摘要：Largemouth bass (Micropterus salmoides) is an important economic freshwater aquaculture species in China, but factors such as disease and a lack of quality seedlings have constrained aquaculture development. In response, we conducted research on breeding for disease resistance and successfully bred a disease-resistant family F1 generation. We used this model to conduct Largemouth bass virus (LMBV) infections to differentiate between resistant and susceptible groups, clarify differences by RNA-sequencing (RNA-seq), and verify disease resistance trait-associated genes using an in vitro infection model. RNA-seq results indicated that pathways such as complement and coagulation cascade, PI3K-Akt, NF-& kgreen;B, and PPAR signaling, and key genes such as C3, PPAR alpha, NFKBIA, JUN, FOS, PI3K, and JAK1 had key roles in LMBV infection resistance. Furthermore, we cloned and preliminarily verified MsPPAR alpha, MsNFKBIA, MsJUN, and MsFOS candidate gene roles in LMBV replication. The results were consistent with RNA-seq data, the low expression of candidate genes effectively inhibited LMBV replication. In conclusion, disease resistance to LMBV is related to lipid metabolism and stabilized inflammatory immune responses. Our study provides a theoretical basis and support for disease-resistance breeding programs for largemouth bass against LMBV infection, and contributes to disease risk and the enrichment of germplasm resources.