文献类型：期刊文献

英文题名：Norethindrone alters mating behaviors, ovary histology, hormone production and transcriptional expression of steroidogenic genes in zebrafish (Danio rerio)

作者：Hou, Liping[1];Chen, Shanduo[1];Shi, Wenjun[2,3,4];Chen, Hongxing[2,3,4];Liang, Yanqiu[8];Wang, Xiaolan[1];Tan, Jiefeng[1];Wang, Yifan[1];Deng, Xikai[1];Zhan, Manjun[1];Long, Jianzhao[1];Cai, Guowei[1];Luo, Shaowen[1];Zhang, Cuiping[1];Liu, Juan[5];Leung, Jonathan Y. S.[6,7];Xie, Lingtian[2,3,4]

机构：[1]Guangzhou Univ, Sch Life Sci, Guangzhou 510655, Peoples R China;[2]South China Normal Univ, Sch Environm, Guangzhou 510006, Peoples R China;[3]South China Normal Univ, Guangdong Prov Key Lab Chem Pollut & Environm Saf, Guangzhou 510006, Peoples R China;[4]South China Normal Univ, Environm Safety & MOE Key Lab Theoret Chem Enviro, Guangzhou 510006, Peoples R China;[5]Minist Educ, Guangdong Prov Key Lab Radionuclides Pollut, Key Lab Water Qual & Conservat Pearl River Delta, Guangzhou, Guangdong, Peoples R China;[6]Southwest Univ, Fac Mat & Energy, Chongqing 400715, Peoples R China;[7]Univ Adelaide, Sch Biol Sci, Adelaide, SA 5005, Australia;[8]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2020

卷号：195

外文期刊名：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

收录：SCI-EXPANDED(收录号：WOS:000525762800037)、、Scopus(收录号：2-s2.0-85081951564)、WOS

基金：This research was funded by the Natural Science Foundation of China (No. 21607032, 41977207, 41877366, 41807352), Fishing Port Construction and Fishery Industry Development (A201701B10), the National Key Research and Development Program of China (2018YFC1801200), Guangdong Province Science Fund for Distinguished Young Scholars (2017KQNCX050), SCNU Scientific Research Fund for Young Teachers (17KJ18), and the Leading Talent Program of South China Normal University to Lingtian Xie.

语种：英文

外文关键词：Reproductive behavior; Norethindrone; Zebrafish; Endocrine disrupting chemical; Steroidogenesis; Gene expression

外文摘要：The impact of progestins (i.e. synthetic forms of progesterone) on aquatic organisms has drawn increasing attention due to their widespread occurrence in the aquatic environments and potential effects on the endocrine system of fish. In this study, the effects of norethindrone (NET, a progestin) on the reproductive behavior, sex hormone production and transcriptional expressions were evaluated by exposing female zebrafish to NET at 0, 3.1, 36.2 and 398.6 ng L-1 for 60 days. Results showed that NET impaired the mating behaviors of female at 36.2 and 398.6 ng L-1 exhibited by males and increased the frequency of atretic follicular cells in the ovary exposed to NET at 398.6 ng L-1. As for sex hormones, plasma testosterone concentration in zebrafish increased, while estradiol concentration decreased. Up-regulation of genes (Npr, Mpra, Mpr beta, Fsh beta, L beta, Tshb, Nis and Dio2) was detected in the brain of fish exposed to NET at 398.6 ng L-1. The transcriptional levels of genes (Esr1, Vtg1, Ar, Cyp19a, Cyp11b and Ptgs2) were generally inhibited in the ovary of zebrafish by NET at 398.6 ng L-1. Moreover, the transcripts of genes (Vtg1, Esr1, Ar and Pgr) in the liver were reduced by NET at 36.2 and 398.6 ng L-1. Our findings suggest that NET can potentially diminish the of fish populations not only by damaging their reproductive organs, but also by altering their mating behavior through the changes in the expressions of genes responsible for the production of sex hormones.