文献类型：期刊文献

英文题名：Identification and expression analysis of cobia (Rachycentron canadum) liver-related miRNAs under hypoxia stress

作者：Huang, Jian-sheng[1,2,3];Li, Hong-juan[1];Guo, Zhi-xiong[1];Zhang, Jian-dong[1,2,3];Wang, Wei-zheng[1];Wang, Zhong-liang[1];Amenyogbe, Eric[1];Chen, Gang[1,2,3]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524025, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhanjian, Zhanjiang 524025, Peoples R China;[3]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China

年份：2021

卷号：47

期号：6

起止页码：1951

外文期刊名：FISH PHYSIOLOGY AND BIOCHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000706008400001)、、WOS

基金：This work was supported by grants from China Agriculture Research System of MOF and MARA (CARS-47) and Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) (ZJW-2019-06).

语种：英文

外文关键词：Rachycentron canadum; Hypoxia stress; miRNAs

外文摘要：At present, due to the influence of global warming, seasonal change, diurnal variation, and eutrophication of the water body, hypoxia has become one of the major factors limiting the stable development of cobia (Rachycentron canadum) culture. In this study, the miRNAs involved in hypoxia stress were screened, and the target genes of miRNAs were annotated and analyzed. The results showed that a total of 184 conservative microRNA (miRNA) and 121 newly predicted miRNA were obtained by sequencing the liver of control (C) and hypoxic (dissolved oxygen, DO (2.64 +/- 0.25) mg/L; 3 h) (S) groups. The pathways involved in energy metabolism included starch and sucrose metabolism (ko00500), glycosaminoglycan degradation (ko00531), and galactose metabolism (ko00052). The results indicate that the body maintains physiological activities by regulating some important pathways at the transcriptional level under hypoxia stress, such as the conversion of aerobic metabolism and anaerobic metabolism, the reduction of energy consumption, and the promotion of red blood cell proliferation to maintain the homeostasis of the body.