文献类型：期刊文献

英文题名：Lycopene alleviates AFB1-induced immunosuppression by inhibiting oxidative stress and apoptosis in the spleen of mice

作者：Xu, Feibo[1,2]; Wang, Peiyan[1,2]; Yao, Qiucheng[3]; Shao, Bing[1]; Yu, Hongyan[1,2]; Yu, Kaiyuan[1,2]; Li, Yanfei[1,2]

机构：[1] College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China; [2] Key Laboratory of the Provincial Education, Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, China; [3] College of Agriculture, Guangdong Ocean University, Zhanjiang, 524000, China

年份：2019

卷号：10

期号：7

起止页码：3868

外文期刊名：Food and Function

收录：EI(收录号：20193007218810)、Scopus(收录号：2-s2.0-85069514318)

语种：英文

外文关键词：Antioxidants - Cell death - Cell proliferation - Mitochondria - Oxidative stress - Proteins

外文摘要：Lycopene (LYC) has been reported to exhibit antioxidant and immunoprotective activities, and our previous studies confirmed that LYC can alleviate multiple tissue damage induced by aflatoxin B1 (AFB1). However, it is unclear whether LYC could relieve the AFB1-induced immunosuppression. Thus, forty-eight male mice were randomly allocated and treated with LYC (5 mg kg-1) and/or AFB1 (0.75 mg kg-1) by intragastric administration for 30 days. We found that LYC alleviated AFB1-induced immunosuppression by relieving splenic structure injury and increasing the spleen weight, spleen coefficient, T lymphocyte subsets, the contents of IL-2, IFN-γ and TNF-α in serum, as well as the mRNA expression of IL-2, IFN-γ and TNF-α in spleen. Furthermore, LYC inhibited oxidative stress induced by AFB1via decreasing the levels of reactive oxygen species (ROS), hydrogen peroxide (H2O2) and malondialdehyde (MDA), while enhancing the total antioxidant capacity (T-AOC) and antioxidant enzyme activities. In addition, LYC also restrained splenic apoptosis through blocking mitochondria-mediated apoptosis in AFB1 intoxicated mice, presenting as the increase of mitochondrial membrane potential, and the decrease of cytoplasmic Cyt-c protein expression, cleaved Caspase-3 protein expression, Caspase-3/9 activities and mRNA expressions, as well as balancing the mitochondrial protein and mRNA expressions of Bax and Bcl-2. These results indicate that LYC can alleviate AFB1-induced immunosuppression by inhibiting oxidative stress and mitochondria-mediated apoptosis of mice spleen. ? 2019 The Royal Society of Chemistry.