文献类型：期刊文献

英文题名：Metabolic Response in the Gill Tissue of Juvenile Black-Shelled Pearl Oyster (Pinctada fucata martensii) under Salinity Stress

作者：Qin, Chengru[1];Lu, Fenglan[1];Li, Junhui[1];Liao, Yongshan[2,3];Yang, Chuangye[1,3,4,5];Deng, Yuewen[1,2,3,4,5]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang 524088, Peoples R China;[3]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[5]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China

年份：2024

卷号：9

期号：9

外文期刊名：FISHES

收录：SCI-EXPANDED(收录号：WOS:001326183400001)、、WOS

基金：This research was funded by National Key Research and Development Program of China (Grant No. 2022YFD2401204), National Natural Science Foundation of China (Grant No. 32102817), Department of Education of Guangdong Province (Grant No. 2020ZDZX1045 and 2021KCXTD026), and the Earmarked Fund for CARS-49.

语种：英文

外文关键词：Pinctada fucata martensii; salinity stress; osmoregulation; metabolomics; gills

外文摘要：Salinity significantly affects shellfish metabolism and growth. In this study, we evaluated the characterization of metabolomic differences in the juvenile black-shelled pearl oyster, Pinctada fucata martensii, under 15 parts per thousand (LSG), 25 parts per thousand (CG), and 35 parts per thousand (HSG) salinity conditions. Non-targeted metabolomics analyses revealed that salinity stress altered the metabolism of pearl oyster. A total of 229 significant differential metabolites (SDMs) were identified between LSG and CG via an in-house MS2 database, 241 SDMs were identified between LSG and HSG, and 50 SDMs were identified between CG and HSG. The pathway analysis showed that 21 metabolic pathways were found between LSG and CG, such as arginine and proline metabolism, glycerophospholipid metabolism, and pentose and glucuronide interconversion. A total of 23 metabolic pathways were obtained between LSG and HSG, such as aspartate, alanine, and glutamate metabolism. Only aminoacyl-tRNA biosynthesis, cysteine and methionine metabolism, and biotin metabolism were enriched between CG and HSG. A further integrated analysis suggested that amino acid metabolism might participate in osmoregulation and energy metabolism to respond to salinity stress in P. f. martensii, and the metabolic pathways differed under varying salinity stress conditions. In addition, low salinity stress might promote apoptosis in pearl oysters. Altogether, these results clarify the salinity tolerance mechanism of pearl oysters.