文献类型：期刊文献

英文题名：Effects of fishmeal replacement by black soldier fly on growth performance, digestive enzyme activity, intestine morphology, intestinal flora and immune response of pearl gentian grouper (Epinephelus fuscoguttatus female x Epinephelus lanceolatus male)

作者：Huang, Bocheng[1];Zhang, Shuang[1,2,3];Dong, Xiaohui[1,2,3];Chi, Shuyan[1,2,3];Yang, Qihui[1,2,3];Liu, Hongyu[1,2,3];Tan, Beiping[1,2,3];Xie, Shiwei[1,2,3,4]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524088, Guangdong, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang 524088, Guangdong, Peoples R China;[3]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Tech, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Guangdong, Peoples R China

年份：2022

卷号：120

起止页码：497

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000740352600003)、、WOS

基金：This work was supported by the fund of The National Key R&D Program of China (2019YFD0900200) and China Agriculture Research System of MOF and MARA (CARS-47) .

语种：英文

外文关键词：Hermetia illucens; Fishmeal replacement; Epinephelus fuscoguttatus female x Epinephelus; lanceolatus male; Digestive enzyme; Intestinal immune response; Intestinal flora

外文摘要：An 8-week feeding trial was conducted to investigate the influence of partial replacement of fishmeal (FM) by black soldier fly (BSF) (Hermetia illucens) on the growth, distal intestine morphology, intestinal flora, and intestinal immune response of pearl gentian grouper (Epinephelus fuscoguttatus female x Epinephelus lanceolatus male). Four diets were formulated, 0% (0 g kg(-1)), 10% (50 g kg(-1)), 20% (100 g kg(-1)) and 30% (150 g kg(-1)) fishmeal were replaced with BSF, named as FM, BSF10, BSF20, BSF30, severally. The study found that, with the increasing dietary BSF levels, growth and feed conversion ratio of fish decreased significantly (P < 0.05). Chitinase and trypsin activities were significantly increased with increasing dietary BSF levels (P < 0.05). With the increasing dietary BSF levels, distal intestinal muscularis thickness and mucosal fold length decreased significantly (P < 0.05), as well as total abundance of intestinal flora. The relative abundance of four phyla and six genera among the top 20 genera were significantly affected by dietary BSF levels (P < 0.05). With the increasing dietary BSF levels, the mRNA levels of nf-kappa bem1, r-cel and il-10 up-regulated significantly (P < 0.05). For fish fed BSF30 diet, the mRNA levels of myd88 and tlr22 were significantly higher than fish fed FM diet (P < 0.05). In conclusion, replacement fishmeal with BSF increased activity of digestive enzymes, but negatively affected growth performance and intestinal health of pearl gentian grouper.