文献类型：期刊文献

英文题名：L-Cysteine hydrochloride inhibits Aspergillus flavus growth and AFB1 synthesis by disrupting cell structure and antioxidant system balance

作者：Qiu, Mei[1]; Qiu, Lihong[2]; Deng, Qi[1]; Fang, Zhijia[1]; Sun, Lijun[1]; Wang, Yaling[1]; Gooneratne, Ravi[3]; Zhao, Jian[4]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang, 524088, China; [2] Department of Clinical Laboratory, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China; [3] Department of Wine, Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, 7647, New Zealand; [4] School of Chemical Engineering, The University of New South Wales, Sydney, NSW, 2052, Australia

年份：2023

卷号：459

外文期刊名：Journal of Hazardous Materials

收录：EI(收录号：20233614695626)、Scopus(收录号：2-s2.0-85169181805)

语种：英文

外文关键词：Aflatoxins - Amino acids - Aspergillus - Biochemistry - Health risks - Transcription

外文摘要：Aflatoxin B1 (AFB1) is the most potent known naturally occurring carcinogen and pose an immense threat to food safety and human health. L-Cysteine hydrochloride (L-CH) is a food additive often used as a fruit and vegetable preservative and also to approved bread consistency. In this study, we investigated the effects and mechanisms of L-CH as an antimicrobial on the growth of Aspergillus flavus (A. flavus) and AFB1 biosynthesis. L-CH significantly inhibited A. flavus mycelial growth, affected mycelial morphology and AFB1 synthesis. Furthermore, L-CH induced glutathione (GSH) synthesis which scavenged intracellular reactive oxygen species (ROS). RNA-Seq indicated that L-CH inhibited hyphal branching, and spore and sclerotia formation by controlling cell wall and spore development-related genes. Activation of the GSH metabolic pathway eliminated intracellular ROS, leading to hyphal dwarfing. L-CH treatment downregulated most of the Aflatoxin (AF) cluster genes and aflS, aflR, AFLA_091090 transcription factors. This study provides new insights into the molecular mechanism of L-CH control of A. flavus and AFB1 foundation. We believe that L-CH could be used as a food additive to control AFB1 in foods and also in the environment. ? 2023