文献类型：期刊文献

中文题名：光合细菌M-01的培养条件优化研究

英文题名：Study on the culture condition of photoautotrophic bacterium M-10

作者：操玉涛[1];陈刚[1];刘立鹤[2];肖明禅[1];叶剑[1];陈佳[1]

机构：[1]湛江海洋大学水产学院,广东湛江524025;[2]湛江水产饲料技术中心,广东湛江524017

年份：2006

卷号：23

期号：1

起止页码：26

中文期刊名：生物学杂志

外文期刊名：Journal of Biology

收录：CSTPCD、、CSCD_E2011_2012、CSCD

语种：中文

中文关键词：光合细菌;分离;正交试验

外文关键词：Photoautotrophie bacterium; screen; orthogonal test

中文摘要：对光合细菌M-01的培养条件进行了优化研究,结果表明:菌株的生长温度为30℃时,最适培养光照为3000lx,最低接种浓度为6%。培养基正交试验研究表明其最优培养基配方为:氯化铵2.00g,磷酸氢二钾0.20g,乙酸钠4.00g,碳酸氢钠2.00g,氯化钠1.00g,酵母膏0.15g,硫酸镁0.20g,T.M储液少量,蒸馏水1000mL;其中乙酸钠为最大影响因子。

外文摘要：The condition of photoautotrophic bacterium M- 10 by liguid culture was studied. The result showed that the optimal condition was temperature 30℃, Light 3000 lux and inoculum 6%. With the orthogonal tests, the medium for M - 10 was obtained. The composition of medium was in one liter containing NH4Cl2.00g, K2HPO4 0.2g, acetate sodium 4.00g, NaHCO3 2.00g, NaCl 1.00g, yeast extract 0.20g, TM a little. As one composition in medium, acetate sodium has stronger ettect on the growth of M- 10.