文献类型：期刊文献

英文题名：A fluorescence method for homogeneous detection of influenza A DNA sequence based on guanine-quadruplex-N-methylmesoporphyrin IX complex and assistance-DNA inhibition

作者：Li, Yubin[1];Liu, Wanshan[1];Zhu, Yinling[1];Diao, Liping[1]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Guangdong, Peoples R China

年份：2019

卷号：91

期号：6

起止页码：979

外文期刊名：JOURNAL OF MEDICAL VIROLOGY

收录：SCI-EXPANDED(收录号：WOS:000465087100011)、、Scopus(收录号：2-s2.0-85061904628)、WOS

基金：Program for scientific research start-up funds of Guangdong Ocean University, Grant/Award Number: R17013; the Non-funded Scientific and Technological Research Projects in Zhanjiang City, Grant/Award Number: 2018B01005; Innovation Strong School Project of Guangdong education department, Grant/Award Number: Q18291

语种：英文

外文关键词：DNA hybridization; fluorescence method; G-quadruplex

外文摘要：In his study, we report a fluorescence method for homogeneous detection of influenza A (H1N1) DNA sequence based on G-quadruplex-NMM complex and assistance-DNA (A-DNA) inhibition. The quadruplex-based functional DNA (QBF-DNA), composed of a complementary probe to the target H1N1 DNA sequence and G-rich fragment, was designed as the signal DNA. The A-DNA consisted of two parts, one part was complementary to target H1N1 DNA and the other part was complementary to the signal DNA. In the absence of target H1N1 DNA, the G-rich fragment of QBF-DNA can form G-quadruplex-NMM complex, which outputted a fluorescent signal. With the presence of target H1N1 DNA, QBF-DNA, and A-DNA can simultaneously hybridize with target H1N1 DNA to form double-helix structure. In this case, the A-DNA partially hybridized with the QBF-DNA, which inhibited the formation of G-quadruplex-NMM complex, leading to the decrease of fluorescent signal. Under the optimum conditions, the fluorescence intensity was inversely proportional to the concentration of target H1N1 DNA over the range from 25 to 700 pmol/L with a detection limit of 8 pmol/L. In addition, the method is target specific and practicability, and would become a new diagnostic assay for H1N1 DNA sequence and other infectious diseases.