文献类型：期刊文献

中文题名：海洋源降解核苷乳酸菌的体外筛选、鉴定及其益生特性研究

英文题名：In vitro screening,identification and probiotic characteristics of marine source lactic acid bacteria with purine nucleoside degrading ability

作者：蚁硕钊[1];李啟彬[1];房志家[1];徐春厚[2];刘颖[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东海洋大学滨海农业学院,广东湛江524088

年份：2022

卷号：48

期号：11

起止页码：87

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：广东省科技计划项目(2016A020222014,2017A030303079)。

语种：中文

中文关键词：高尿酸血症;核苷;乳酸菌;降解率;益生特性

外文关键词：hyperuricemia;nucleoside;lactic acid bacteria;degradation rate;probiotic properties

中文摘要：乳酸菌具有降解肠道中核苷的能力,已成为预防或辅助治疗高尿酸血症的有效方法。以肌苷、鸟苷、腺苷作为降解底物,采用HPLC法从海洋动物肠道中筛选降解核苷乳酸菌,并对降解菌株的益生特性进行研究。结果表明,在分离到的30株菌株中,菌株BX-59与2Y-15对3种核苷的降解能力最强,经鉴定为发酵粘液乳杆菌(Limosilactobacillus fermentum)。其特性实验表明,2株发酵粘液乳杆菌不具有溶血性,对抗生素无耐药性,同时对DPPH与·OH的清除率分别为61.05%、58.36%与57.84%、61.12%。2株菌在人工胃液与肠液中的存活率分别为98.7%、99.8%与80.0%、64.0%。菌株BX-59在人工饱腹肠液环境状态下,对肌苷、鸟苷、腺苷降解率为60.78%、51.34%、59.00%,而菌株2Y-15在人工饱腹肠液环境状态下,对肌苷、腺苷的降解率为100%,对鸟苷的降解率为98.60%。该研究为2株菌作为防治高尿酸血症微生态菌剂的开发与应用提供依据。

外文摘要：Lactic acid bacteria can degrade nucleosides in the intestinal tract and have become an effective method to prevent or assist in the treatment of hyperuricemia.In this study,the nucleoside-degrading lactic acid bacteria were screened with inosine,guanosine,and adenosine as substrates,and the probiotic properties of the degrading strains were studied.The results showed that strain BX-59 and strain 2Y-15 had the strongest degrading ability and were identified as Limosilactobacillus fermentum.The characteristic experiment shows that the two L.fermentum were not hemolytic or resistant to the tested antibiotics.At the same time,the scavenging rates of 1,1-diphenyl-2-picryl-hydrazyl(DPPH)and hydroxyl free radicals were 61.05%,58.36%,57.84%,and 61.12%,respectively.The survival rate of the two strains in the artificial gastric and intestinal fluids were 98.7%,99.8%and 80.0%,64.0%,respectively.Under the influence of simulated intestinal fluid and nutrients,the strain BX-59 exhibited 60.78%,51.34%,and 59.00%degradation of inosine,guanosine,and adenosine,respectively.While the strain 2Y-15 exhibited 100%degradation of inosine and adenosine,and 98.60%degradation of guanosine in the environment of artificial satiety intestinal fluid.This study provides the basis for the development and application of two strains regard as microecological agents for the prevention and treatment of hyperuricemia.